[关键词]
[摘要]
目的 明确四妙方防治非酒精性脂肪肝病(non-alcoholic fatty liver disease,NAFLD)的作用,并比较四妙方颗粒剂与汤剂中主要成分质量分数与防治NAFLD的药效差异。方法 HPLC检测四妙方颗粒剂与汤剂中黄柏碱、木兰花碱、β-蜕皮甾酮、药根碱、巴马汀与小檗碱的质量分数。将C57BL/6小鼠随机分为对照组,模型组,四妙方颗粒剂低、高剂量(10、20 g/kg)组,四妙方汤剂低、高剂量(10、20 g/kg)组,通过高糖高脂饮食建立NAFLD小鼠模型,给予四妙方颗粒剂与汤剂进行干预,检测各组小鼠的体质量、肝脏质量、附睾脂肪质量和血清脂质、肝脏脂质水平;采用苏木素-伊红(HE)染色观察各组小鼠肝组织病理变化。结果 四妙方汤剂中6种成分的质量分数均高于颗粒剂,四妙方全方的色谱峰主要来源于黄柏,其余药味的色谱峰较少。与对照组比较,模型组小鼠体质量、肝脏质量、附睾脂肪质量和血清中总胆固醇(total cholesterol,TC)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、尿酸水平及丙氨酸转氨酶(alanine aminotransferase,ALT)、天冬氨酸转氨酶(aspartate aminotransferase,AST)活性及肝脏中三酰甘油(triglycerides,TG)水平均显著升高(P<0.05、0.01),且脂肪严重变性。与模型组比较,四妙方颗粒剂与汤剂均能有效降低小鼠体质量、肝脏质量、附睾脂肪质量及血清中TC、LDL-C、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、尿酸水平及AST、ALT活性及肝脏中三酰甘油(triglycerides,TG)水平(P<0.05、0.01),且汤剂低剂量组药效优于颗粒剂低剂量组;四妙方颗粒剂与汤剂均能减少脂质聚积、脂肪变性,汤剂低剂量组药效优于颗粒剂低剂量组。结论 四妙方汤剂中的化学成分质量分数高于颗粒剂,低剂量下汤剂药效优于颗粒剂,高剂量下颗粒剂与汤剂差异较小。
[Key word]
[Abstract]
Objective To verify the effect of Simiao Formula (四妙方) on non-alcoholic fatty liver disease (NAFLD), and study the difference of ingredients and efficacy between Simiao Formula Granules (四妙方颗粒) and Decoction on NAFLD. Methods The contents of phellodendrine, magnoflorine, 20-hydroxyecdysone, jatrorrhizine, palmatine, and berberine in Simiao Formula Granules and Simiao Decoction were detected by HPLC. Male C57BL/6 mice were randomly divided into control group, model group, low-, high-dose Simiao Granules (10, 20 g/kg), low-, high-dose Simiao Decoction (10, 20 g/kg). Mice received a high fat/high sucrose diet to induce NAFLD. The body weight, liver weight, epididymal white adipose tissue (eWAT) weight, serum lipids, and liver lipids of each group were detected. HE staining was used to observe the pathology of liver tissue. Results The results of HPLC showed that the contents of six components in decoction were higher than those in granules. The main chromatographic peaks of Simiao Formula came from Phellodendri Chinensis Cortex, few peaks come from the rest of the medicine. The results of pharmacodynamics showed that the body weight, liver weight, eWAT weight, serum total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), uric acid, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and liver triglycerides (TG) of the model group were significantly increased compared with the control group (P<0.05, 0.01). HE showed severe steatosis in model group. Compared with model group, both Simiao Formula Granules and Decoction effectively reduced the body weight, liver weight, eWAT weight, serum TC, LDL-C, HDL-C, uric acid, AST, ALT and liver TG of the mice (P<0.05, 0.01). The efficacy in the low dosage decoction group were better than that in the low dosage granules. Liver pathology showed that both Simiao Formula Granules and the Decoction significantly reduced lipid accumulation and steatosis, but the low dosage decoction group was significantly better than the low dosage granule group. Conclusion The content of chemical components in Simiao Formula Decoction was higher than that in Granules. The efficacy of Simiao Formula Decoction was better than that of Simiao Formula Granules at low dosage, but the difference was small at high dosage.
[中图分类号]
R285.5
[基金项目]
国家重点研发计划项目(2017YFC1700200);上海市卫健委中医药科研项目(2020JP016)