[关键词]
[摘要]
目的 研究生附子Aconitum carmichaelii内生真菌Trichoderma sp.FZ-31的化学成分。方法 内生真菌发酵物采用正相硅胶柱色谱、反相硅胶柱色谱、Sephadex LH-20凝胶柱色谱及制备型高效液相色谱等方法进行分离纯化,再通过核磁共振、质谱以及圆二色谱等方法鉴定单体化合物结构。结果 从Trichoderma sp.FZ-31发酵产物的醋酸乙酯部位得到11个化合物,分别鉴定为(-)-蜂蜜曲菌素(1)、(R)-7-羟基蜂蜜曲菌素(2)、4-羟基蜂蜜曲菌素(3)、赤霉菌吡喃酮D(4)、阿魏酸甲酯(5)、吲哚-3-乙酸(6)、吲哚-3-甲醛(7)、苯甲酰胺(8)、(3R,6R)-4-甲基-6-(1-甲基乙基)-3-苯甲基全氢-1,4-噁嗪-2,5-二酮(9)、酒渣碱(10)、腐皮壳素A(11)。结论 除化合物5、7外,其余化合物均为首次从木霉菌Trichoderma sp.中分离得到,本研究为附子及其内生菌的开发利用提供了一定的研究基础。
[Key word]
[Abstract]
Objective To study the chemical constituents of endophytic fungi Trichoderma sp. FZ-31 from Aconitum carmichaelii. Methods The fermentation of Trichoderma sp. FZ-31 was isolated and purified through silica gel, Sephadex LH-20 as well as preparative HPLC, and the structures of obtained compounds were identified by NMR, MS, and circular dichroism. Results Eleven compounds were isolated and identified as (-)-mellein (1), (R)-7-hydroxymellein (2), 4-hydroxymellein (3), gibepyrone D (4), methyl ferulate (5), indole-3-acetic acid (6), indol-3-carbaldehyde (7), benzamide (8), (3R,6R)-4-methyl-6-(1-methylethyl)-3-phenylmethylperhydro-1,4-oxazine-2,5-dione (9), flazine (10), and diaporthein A (11). Conclusion Except compounds 5 and 7, the remain compounds are isolated from Trichoderma sp. for the first time. This study provides a certain study basis for A. carmichaelii and its endophytic fungi.
[中图分类号]
R284.1
[基金项目]
国家重点研发计划(2017YFE0119900);国家自然科学基金面上项目(81874343)