目的 研究紫花地丁Viola yedoensis全草的化学成分。方法 采用硅胶、ODS、Sephadex LH-20凝胶和半制备型高效液相等柱色谱方法进行分离纯化，通过理化性质结合NMR、MS等谱学数据分析鉴定化合物结构。结果 从紫花地丁95%乙醇提取物中分离得到21个化合物，分别鉴定为秦皮乙素-6-O-β-D-呋喃芹糖基-（1→2）-β-D-吡喃葡萄糖苷（1）、吲哚-3-甲酸乙酯（2）、脱氢地芰普内酯（3）、滨蒿内酯（4）、蒲公英苦素（5）、水杨苷（6）、丁香苷（7）、4-羟基-4-[3'-（β-D-葡萄糖基）亚丁基]-3，5，5-三甲基-2-环己烯-1-醇（8）、柑橘苷A（9）、（7S，8R）-二氢去氢二松柏醇-9-O-β-D-吡喃葡萄糖苷（10）、山柰素-3-O-α-L-（4-O-乙酰基）鼠李糖基-7-O-α-L-鼠李糖苷（11）、山柰酚-7-O-α-L-鼠李糖苷（12）、山柰酚-3-O-α-L-鼠李糖苷（13）、山柰酚-3-O-α-L-鼠李糖基-7-O-α-L-鼠李糖苷（14）、山柰酚-3-O-β-D-葡萄糖苷（15）、芹菜素（16）、（+）-异地芰普内酯（17）、尿苷（18）、腺苷（19）、6-羟基-香豆素-7-O-α-L-鼠李糖基-（1→6）-O-β-D-葡萄糖苷（20）、（7S，8R）-二氢去氢二松柏醇-4-O-β-D-吡喃葡萄糖苷（21）。结论 化合物1为新化合物，命名为紫丁葡芹苷，2为新天然产物，5～11和18为首次从堇菜属中分离得到，12和21为首次从紫花地丁中分离得到。

Objective To investigate the chemical constituents from whole herbs of Viola yedoensis. Methods The chemical constituents were isolated and purified by silica gel, ODS column chromatography, Sephadex LH-20 column chromatography and semi-preparative HPLC. The structures of the isolated compounds were identified by physicochemical properties, NMR and MS spectroscopic methods. Results A total of 21 compounds were isolated from the 95% ethanol extract of V. yedoensis, and their structures were identified as aesculetin 6-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside (1), ethyl indole-3-carboxylate (2), dehydrololiolide (3), scoparone (4), taraxacin (5), salicin(6), syringin (7), (E)-4-hydroxy-4-[3'-(β-D-glucopyranosyloxy) butylidene]-3,5,5-trimethyl-2-cyclohexen-l-one (8), citroside A (9), (7S,8R)-dihydrodehydrodiconiferyl alcohol-9-O-β-D-glucopyranoside (10), 3-O-α-L-(4-O-acetyl)rhamnopyranosyl kaempferol 7-O-α-L-rhamnopyranoside (11), kaempferol-7-O-α-L-rhamnopyranoside (12), kaempferol-3-O-α-L-rhamnopyranoside (13), kaempferitrin (14), kaempferol-3-O-β-D-glucopyranoside (15), apigenin (16), (+)-isololiolide (17), uridine (18), β-adenosine (19), 6-hydroxycoumarin-7-O-α-L-rhamnosyl-(1→6)-O-β-D-glucoside (20), and (7S,8R)-dihydrodehydrodiconiferyl alcohol 4-O-β-D-glucopyranoside (21). Conclusion Compound 1 is obtained as a new compound, named as vioyegluapiside and compound 2 as a new natural product, with compounds 5-11 and 18 isolating from the genus Viola for the first time and with compounds 12 and 21 from plant V. yedoensis for the first time.

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山东省重点研发计划（鲁渝科技协作）项目（2019LYXZ026）；山东省农业科技园区产业提升工程项目（2019YQ033）；山东第一医科大学学术提升计划（2019LJ003）