目的 研究太子参Pseudostellaria heterophylla内生真菌草酸青霉Penicillium oxalicum的次级代谢产物。方法 采用正、反相硅胶柱色谱，葡聚糖凝胶Sephadex LH-20柱色谱以及制备薄层色谱（pTLC）等色谱技术进行系统分离纯化，采用质谱（MS）、核磁共振（NMR）等波谱学手段对单体化合物进行结构鉴定。结果 从草酸青霉发酵产物的醋酸乙酯萃取液中分离鉴定了12个化合物，包括1个香豆酮类化合物5，7-二羟基-2-甲基苯并呋喃-3-羧酸（1）、5个异香豆素类化合物（3R，4R）-（-）-4-hydroxymellein（2）、O-methylmellein（3）、acremonone G（4）、decarboxycitrinone（5）和decarboxyhydroxycitrinone（6），4个没药烷型倍半萜类化合物（7S，11S）-（+）-12-acetoxysydonic acid（7）、（S）-（+）-11-dehydrosydonic acid（8）、sydonic acid（9）和1-hydroxy-boivinianin A（10），2个混源萜类化合物decaturin D（11）和decaturin C（12）。结论 化合物1为新化合物，命名为草酸苯并呋喃酸A，化合物2～6、10为首次从草酸青霉中分离得到。

Objective To study the secondary metabolites of Penicillium oxalicum, an endophytic fungus isolated from the medicinal plant Pseudostellaria heterophylla. Methods The secondary metabolites were isolated by silica gel, Sephadex LH-20 column chromatographies, and prep-TLC methods. Their structures were elucidated by using various spectroscopic techniques including HRESIMS and NMR spectra. Results A benzofuran compound 5,7-dihydroxy-2-methylbenzofuran-3-carboxylic acid (1), five isocoumarins including (3R,4R)-(-)-4-hydroxymellein (2), O-methylmellein (3), acremonone G (4), decarboxycitrinone (5) and decarboxyhydroxycitrinone (6), four bisabolane-type sesquiterpenoids including (7S,11S)-(+)-12-acetoxysydonic acid (7), (S)-(+)-11-dehydrosydonic acid (8), sydonic acid (9) and 1-hydroxy-boivinianin A (10), as well as two meroterpenoid-type alkaloids decaturin D (11) and decaturin C (12) were isolated. Conclusion Compound 1 is a new benzofuran compound and named as oxafuranone A, while compounds 2—6 and 10 are characterized from P. oxalicum for the first time.

R284.1