目的 探讨并验证中药和颜^®坤泰胶囊（HKC）发挥抗氧化作用的潜在机制。方法 通过中药系统药理学数据库与分析平台（TCMSP）探索HKC中21种成分发挥作用的靶基因，再利用数据库（DAVID）对这些靶基因进行基因本体（GO）富集和京都基因和基因组百科全书（KEGG）信号通路分析；通过体外构建H₂O₂诱导的MII卵母细胞氧化应激模型对分析结果进行验证。结果 HKC的21个成分共对应151个靶基因，GO富集结果主要集中在线粒体功能、超氧化物歧化酶以及能量代谢途径，而KEGG结果富集在PI3K/AKT、ErbB1、mTOR等信号通路。体外实验结果证实在氧化应激的MII卵母细胞中，HKC通过改善线粒体功能，增加ATP的产生，同时也减少线粒体膜电势的降低发挥抗氧化作用。结论 HKC通过改善线粒体功能发挥抗氧化作用。

Objectives To explore and verify the potential antioxidation mechanism of traditional Chinese medicine Heyan Kuntai Capsule (HKC). Methods Gene targets of 21 ingredients of HKC were searched for through Traditional Chinese Medicine Systems Pharmacology (TCMSP) database and analysis platform, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis with Database for Annotation, Visualization and Integrated Discovery (DAVID) tools. Further, the anti-oxidative function of HKC was validated in an in vitro H₂O₂-induced oxidative stress model in MII oocytes. Results A total of 151 genes were obtained as targets to 21 components of HKC, whose GO were further significantly enriched in mitochondrion, superoxide dismutase activity and energy pathways, while KEGG showed the enrichment in PI3K/AKT, mTOR and ErbB1 signaling pathways. Afterwards, it was confirmed that HKC increased ATP production in H₂O₂-treated MII oocytes by improving mitochondrial function and reduced the dissipation of mitochondrial membrane potential (ΔΨ_m) to exert the antioxidation effect. Conclusions HKC exerts anti-oxidative effect by improving mitochondrial function.

R285.5