目的 通过观察异常山碱对食管癌EC9706细胞的增殖、凋亡、周期、能量代谢及能量代谢通路相关蛋白表达的影响，探讨其治疗食管癌的分子机制。方法 常规培养ECC9706细胞，用MTT法检测细胞活性，筛选药物浓度，选择1、2 μg/mL 2个质量浓度；流式细胞术检测异常山碱对EC9706细胞凋亡、周期的影响，能量代谢检测系统检测异常山碱对EC9706细胞能量代谢的影响，Western blotting检测细胞中哺乳动物雷帕霉素靶蛋白（mTOR）、磷酸化哺乳动物雷帕霉素靶蛋白（p-mTOR）、磷酸化乙酰辅酶A羧化酶（p-ACC）、腺苷磷酸激活蛋白激酶（AMPK）蛋白表达。结果 不同质量浓度异常山碱作用48 h后能有效抑制EC9706细胞增殖，呈剂量依赖性（P<0.01），可以将EC9706细胞阻滞于S期和G₂/M期（P<0.05），有效促进细胞凋亡（P<0.05），且明显抑制细胞糖酵解及线粒体代谢能力（P<0.01），与对照组比较，异常山碱组细胞AMPK蛋白表达水平上升，mTOR、p-mTOR、p-ACC蛋白表达水平降低（P<0.05）。结论 异常山碱可能通过能量代谢调节EC9706细胞周期、凋亡，抑制EC9706细胞增殖。

objective To investigate the molecular mechanism of isofebrifuzine in the treatment of esophageal cancer by observing the effects of isofebrifuzine on proliferation, apoptosis, cycle, energy metabolism and protein expression related to energy metabolism pathway in EC9706 cells. Methods ECC9706 cells were routinely cultured, cell activity was detected by MTT method, drug concentration was screened, and two concentrations of 1 μg/mL and 2 μg/mL were selected, the effect of isofebrifuzine on apoptosis and cycle of esophageal cancer cells EC9706 was detected by flow cytometry. The effect of isofebrifuzine on energy metabolism of EC9706 cells was detected by energy metabolism detection system, and the protein expressions of mTOR, p-mTOR, p-ACC and AMPK in cells were detected by Western blotting. Results The proliferation of EC9706 cells was effectively inhibited in a dose-dependent manner (P<0.01) after 48 h of treatment with different concentrations of isofebrifuzine, which could arrest EC9706 cells in S phase and G₂/M phase (P<0.05), effectively promote cell apoptosis (P<0.05), and significantly inhibit cell glycolysis and mitochondrial metabolism (P<0.01). Compared with the control group, AMPK expression was increased and mTOR, p-mTOR, p-ACC expression was decreased in the treatment group (P<0.05). Conclusion These results indicated that isofebrifuzine may regulate the cycle and apoptosis of EC9706 cells and inhibit the proliferation of EC9706 cells in esophageal cancer through energy metabolism.

R285.5

国家自然科学基金面上项目（81173177）；国家自然科学基金面上项目（81373535）；河南省高等学校重点科研项目（19A3600-14）