目的 研究滇重楼内生真菌米曲霉Aspergillus oryzae次生代谢产物的化学成分，以期发现新的化合物。方法 采用液体发酵方式发酵，萃取后运用硅胶及大孔吸附树脂等多种色谱技术进行分离纯化，并根据理化性质和波谱数据鉴定化合物的结构。结果 从滇重楼内生真菌米曲霉发酵产物中分离鉴定了3个化合物，分别鉴定为3-氨基-4，5-二羟基-4，6-二甲基-2-（2-甲基丁酰）环己-2-烯酮（1）、12-N-甲基-环-（L-色氨酰-L-苯丙氨酰）（2）和二聚色氨酸素（3）。结论 化合物1为新的聚酮类化合物，命名为米曲霉聚酮A。

Objective To study the secondary metabolites of the endophytic fungus Aspergillus oryzae from Paris polyphylla var. yunnanensis in order to find new compounds. Methods The endophytic fungus A. oryzae was fermented by liquid fermentation. After extraction, silica gel and macroporous adsorption resin were used to separate and purify the extract. The structures of the compounds were identified according to their physical and chemical properties and spectroscopic data. Results Three compounds were isolated and their structures were identified as 3-amino-4,5-dihydroxy-4,6-dimethyl-2-(2-methylbutanoyl)cyclohex-2-enone (1), 12-N-methyl-cyclo-(L-tryptophyl-L-phenylalanyl) (2) and ditryptophenaline (3). Conclusion Compound 1 is a new polyketide named asperpolyketide A.

R284.1

国家自然科学基金项目（31560099）；国家自然科学基金项目（31860099）；云南省高校科技创新团队支持计划；云南民族大学化学学科博士点培育项目（BSD-HX-2019-20）