目的 研究桃儿七Sinopodophyllum hexandrum中黄酮类化学成分。方法 采用正相硅胶柱色谱、ODS-C₁₈柱色谱、Sephadex LH-20凝胶柱色谱、制备薄层色谱等方法进行分离和纯化，通过波谱数据鉴定化合物的结构，采用MTT法研究化合物的细胞毒活性。结果 从桃儿七95%乙醇冷浸提取物的醋酸乙酯部位分离出9个化合物，分别鉴定为8，2'-二异戊烯基槲皮素-3-甲醚-4'-O-β-D-葡萄糖苷（1）、8，2'-diprenylquercetin-3-methylether（2）、5，7，4'-trihydroxy-3'-（3-methylbut-2-enyl）-3-methoxy flavone（3）、8-prenylkaempferol（4）、sophoflavescenol（5）、podoverine A（6）、sinoflavonoid K（7）、香叶木素（8）、金合欢素（9）。结论 化合物1为未见文献报道的异戊烯基黄酮苷类化合物，命名为桃儿七酮苷A；化合物5~9为为首次从该植物中分离得到。细胞毒活性结果显示，化合物1~5对人宫颈癌HeLa细胞均有一定的抑制作用，其半数抑制浓度（IC₅₀）值分别为42.6、46.9、26.9、16.1、31.2 μmol/L。

Objective To study the chemical constituents from Sinopodophyllum hexandrum and their antitumor activities. Methods The constituents were separated by chromatography of silica gel, ODS, Sephadex LH20 and pre-TLC. Their structures were elucidated by spectroscopic means. The in vitro cytotoxic activities of the isolated compounds were studied by MTT method. Results Nine compounds were isolated and identified as 8,2'-diprenylquercetin 3-methyl ether-4'-O-β-D-glucoside (1), 8,2'-diprenyl quercetin-3-methylether (2), 5,7,4'-trihydroxy-3'-(3-methylbut-2-enyl)-3-methoxy flavone (3), 8-prenylkaempferol (4), sophoflavescenol (5), podoverine A (6), sinoflavonoid K (7), diosmetin (8) and acacetin (9). Conclusion Compound 1 is a new compound named sinoflavonoid glycosides A, and compounds 5-9 are isolated from S. hexandrum for the first time. Compounds 1-5 show cytotoxicities against HeLa cells with IC₅₀ of 42.6, 46.9, 26.9, 16.1 and 31.2 μmol/L, respectively.

R284.1