目的 研究麦冬皂苷D（ophiopogonin D，OP-D）对高脂饲养ApoE^-/-小鼠血脂及肠道菌群的影响。方法 选取6周龄雄性ApoE^-/-小鼠24只，随机均分为对照组、模型组、OP-D组［0.5 mg/（kg·d）］和辛伐他汀组［5 mg/（kg·d）］；雄性C57BL/6小鼠6只为空白组。造模12周后，连续给药12周。给药结束后收集小鼠新鲜粪便，检测菌群情况；分离小鼠血清检测血脂水平，制备肝脏切片染色观察损伤情况。结果 OP-D能够降低小鼠因高脂饮食引起的体质量增加，抑制总胆固醇（TC）和三酰甘油（TG）升高，改善肝脂肪变性，并多种水平上调控肠道菌群失调。结论 OP-D可能通过改善高脂饲养的ApoE^-/-小鼠肠道菌群失调影响其血脂水平和肝脂肪变性。

Objective To investigate the effect of ophiopogonin D (OP-D) on blood lipids and intestinal flora in ApoE^-/- mice fed with high-fat diet (HFD). Methods A total of 24 male ApoE^-/- mice, aged six weeks old, were randomly divided into control group, model group, OP-D group[0.5 mg/(kg∙d)] and simvastatin group[5 mg/(kg∙d)]. Another six male C57BL/6 mice were in blank group. After 12 weeks of HFD, the drugs were given by intragastric administration for 12 weeks. After the end of administration, fresh feces of mice were collected to detect intestinal flora. Serum of mice was separated to detect blood lipid. Liver section staining was prepared to observe the damage. Results OP-D could reduce the weight gain of mice caused by HFD, inhibit the increase of total cholesterol and triglyceride, improve hepatic steatosis, and regulate intestinal flora imbalance. Conclusion OP-D may regulate blood lipids and hepatic steatosis by improving intestinal flora imbalance induced by HFD in ApoE^-/- mice.

R285

国家自然科学基金资助项目（81774213）；南方医科大学大学生创业创新训练计划项目（201812121009）；南方医科大学大学生创业创新训练计划项目（201812121140）