[关键词]
[摘要]
目的 研究东北岩高兰Empetrum nigrum var. japonicum的化学成分。方法 采用溶剂萃取、硅胶柱色谱、高效液相色谱等方法进行分离纯化,通过理化性质及波谱数据分析鉴定结构。结果 从东北岩高兰醋酸乙酯萃取物中分离得到22个化合物,分别鉴定为3,4-二羟基苯甲酸(1)、3-甲氧基-4-羟基苯甲酸(2)、3,4-二羟基苯甲酸甲酯(3)、二氢松柏醇(4)、(6S,9R)-6,9-二羟基-3-酮-α-紫罗兰醇(5)、(6R,9R)-9-羟基-3-酮-α-紫罗兰醇(6)、苯丙酸(7)、槲皮素3-O-(6″-苯甲酰)-β-D-吡喃半乳糖苷(8)、胡萝卜苷(9)、槲皮素-3-O-β-D-吡喃葡萄糖苷(10)、苯丙醇-O-β-D-吡喃葡萄糖苷(11)、山楂酸(12)、芦丁(13)、槲皮素-3-O-β-D-吡喃半乳糖苷(14)、苯甲醇-O-(6-O-α-L-呋喃阿拉伯糖基)-β-D-吡喃葡萄糖苷(15)、肉桂醇-O-(6-O-α-L-呋喃阿拉伯糖基)-β-D-吡喃葡萄糖苷(16)、槲皮素-3-O-(2-O-α-L-吡喃鼠李糖基)-β-D-吡喃葡萄糖苷(17)、山柰酚-3-O-(2-O-α-L-吡喃鼠李糖基)-β-D-吡喃葡萄糖苷(18)、2α,3β-二羟基-12-烯-28-乌苏酸(19)、foliachinenoside A2(20)、山柰酚-3-O-芸香糖苷(21)、异鼠李素-3-O-芸香糖苷(22)。结论 化合物1~6、8、11~13、15~22为首次从东北岩高兰中分离得到。
[Key word]
[Abstract]
Objective To study the chemical constituents of Empetrum nigrum var. japonicum. Methods The chemical constituents were isolated by silica gel column chromatography and HPLC, and its structure was identified by their spectral data and physicochemical properties analysis. Results Twenty-two compounds were isolated from ethyl acetate extracts of E. nigrum var. japonicum with the structures identified as 3,4-dihydroxybenzoic acid (1), 3-methoxy-4-hydroxybenzoic acid (2), methyl-3,4-dihydroxybenzoate (3), dihydroconiferyl alcohol (4), (6S,9R)-vomifoliol (5), (6R,9R)-9-hydroxy-3-one-α-ionol (6), phenylpropionic acid (7) quercetin-3-O-(6″-benzoyl)-β-D-galactoside (8), daucosterol (9), quercetin-3-O-β-D-glucopyranoside (10), phenylpropyl-O-β-D-glucopyranoside (11), maslinic acid (12), rutinum (13), quercetin-3-O-β-D-galactoside (14), benzyl-(6-O- α-L-arabinofuranosyl)-O-β-D-glucopyranoside (15), cinnamol-O-(6-O-α-L-arabinopyranosyl)-β-D-glucopyranoside (16), quercetin-3- O-neohesperidoside (17), kaempferol-3-O-neohesperidoside (18), 2α,3β-dihydroxyurs-12-en- 28-oic acid (19), foliachinenoside A2 (20), kaempferol-3-O-rutinoside (21) and isorhamnetin-3-O-rutinoside (22). Conclusion Compounds 1-6, 8, 11-13, 15-22 were isolated from E. nigrum var. japonicum for the first time.
[中图分类号]
R284.1
[基金项目]
黑龙江省属高等学校基本科研业务费项目(YSTSXK201808);内蒙古自治区自然科学基金资助(2017MS0212);齐齐哈尔大学研究生创新科研项目(YJSCX2018-018X);呼伦贝尔市科技计划项目(2016.01)