[关键词]
[摘要]
目的 研究一枝蒿Artemisia rupestris干燥带根全草的化学成分。方法 采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱及半制备高效液相色谱法分离纯化;通过核磁共振、质谱等波谱数据分析鉴定化合物的结构。结果 从一枝蒿全草70%乙醇提取物的石油醚和醋酸乙酯萃取物中共分离得到18个化合物,分别鉴定为2α-(2',4'-hexadiynoyl)-1,6-dioxaspiro[4,5]-deca-3-ene(1)、大黄酚(2)、香草酸乙酯(3)、亚麻酸甲酯(4)、8,11-十八碳二烯酸甲酯(5)、香豆酸二十烷酯(6)、大黄素(7)、2,5-二叔丁基苯酚(8)、珊瑚菜内酯(9)、紫丁香酸(10)、7-甲氧基香豆素(11)、香草醛(12)、欧前胡素(13)、千层纸素A(14)、5-羟基-3,4',6,7-四甲氧基黄酮(15)、芥酸酰胺(16)、octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propanoate(17)和3,5,3',4'-四羟基-6,7-二甲氧基黄酮(18)。结论 除化合物1、4、11~14和18为首次从该植物中分离得到外,其余化合物均为首次从蒿属植物中分离得到。
[Key word]
[Abstract]
Objective To investigate the chemical constituents of the whole plants of Artemisia rupestris. Methods The chemical constituents were isolated and purified by silica gel, Sephadex LH-20 column chromatography and semi-preparative HPLC. The structures of the isolated compounds were identified by NMR and MS spectroscopic method. Results Eighteen compounds were isolated and purified, which structures were identified as 2α-(2',4'-hexadiynoyl)-1,6-dioxaspiro[4,5]-deca-3-ene (1), chrysophanol (2), ethyl vanillate (3), methyl linolenate (4), 8,11-octadecadienoic acid methyl ester (5), eicosane coumaric acid ester (6), emodin (7), 2,5-di-tert-butylphenol (8), phellopterin (9), syringic acid (10), 7-methoxycoumarin (11), vanillin (12), imperatorin (13), oroxylin A (14), 5-hydroxy-3,4',6,7-tetramethoxyflavone (15), erucylamide (16), octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propanoate (17) and 3,5,3',4'-tetrahydroxy-6,7-dimethoxyflavone (18). Conclusion Compounds 1, 4, 11-14 and 18 are isolated from A. rupestris for the first time, and all the other compounds were isolated from the genus Artemisia for the first time.
[中图分类号]
R284.1
[基金项目]
国家自然科学基金面上项目(81473421)