目的 建立盐黄柏饮片和易黄汤的特征图谱与主要成分测定方法。方法 采用HPLC法建立盐黄柏饮片和易黄汤的特征图谱，并同时测定4种主要成分（盐酸黄柏碱、4-O-阿魏酰奎尼酸、5-O-阿魏酰奎尼酸和盐酸小檗碱）的含量。结果 以10批盐黄柏饮片及易黄汤为研究对象，建立了相应的HPLC特征图谱，确定了5个共有峰，并对其中的4个共有峰（盐酸黄柏碱、4-O-阿魏酰奎尼酸、5-O-阿魏酰奎尼酸和盐酸小檗碱）进行了化学成分的确认和含量测定，发现盐黄柏饮片中盐酸黄柏碱、4-O-阿魏酰奎尼酸、5-O-阿魏酰奎尼酸和盐酸小檗碱的质量分数分别在0.841%~1.314%、0.358%~1.841%、2.495%~5.498%、4.259%~7.007%；易黄汤中盐酸黄柏碱、4-O-阿魏酰奎尼酸、5-O-阿魏酰奎尼酸、盐酸小檗碱的质量浓度分别在69.71~117.80、107.85~165.79、252.96~348.20、213.61~361.45 μg/mL，易黄汤中以盐黄柏饮片的质量为基准折算后的盐酸黄柏碱、4-O-阿魏酰奎尼酸、5-O-阿魏酰奎尼酸和盐酸小檗碱的质量分数分别为0.509%~0.865%、0.788%~1.212%、1.849%~2.545%、1.561%~2.655%。结论 建立的测定方法不仅为盐黄柏饮片和易黄汤的质量控制提供了更加完善的参考和依据，也为盐黄柏饮片和易黄汤的效应物质基础研究提供了一定的基础。

Objective To establish a method for the characteristic chromatogram and determination of indicative components of salt-fried Phellodendri Chinensis Cortex (sPCC) and Yihuang Decoction (YHD). Methods An HPLC was established for characteristic chromatogram analysis and determination of four indicative components (phellodendrine, 4-O-feruloylquinic acid, 5-O-feruloylquinic acid and berberine) of sPCC and YHD. Results The characteristic chromatogram of sPCC and YHD were established by HPLC from 10 batches. Among five common peaks, four indicative components (phellodendrine, 4-O-feruloylquinic acid, 5-O-feruloylquinic acid and berberine) were identified and determined. The mass fraction of phellodendrine, 4-O-feruloylquinic acid, 5-O-feruloylquinic acid and berberine in sPCC ranged from 0.841%-1.314%, 0.358%-1.841%, 2.495%-5.498%, and 4.259%-7.007%, respectively; The mass concentration of phellodendrine, 4-O-feruloylquinic acid, 5-O-feruloylquinic acid and berberine in YHD ranged from 69.71-117.80, 107.85-165.79, 252.96-348.20, and 213.61-361.45 μg/mL, respectively; and the mass fraction of phellodendrine, 4-O-feruloylquinic acid, 5-O-feruloylquinic acid and berberine converted from YHD based on the mass of sPCC ranged from 0.509%-0.865%, 0.788%-1.212%, 1.849%-2.545%, and 1.561%-2.655%, respectively. Conclusion The established method not only provides more complete reference and basis for the quality control, but also provides a certain basis for the research of effect substance basis of sPCC and YHD.

R286.02

江苏省中药资源产业化过程协同创新中心项目（ZDXM-1-3）；江苏高校优势学科建设工程资助项目（PAPD）