目的 初步筛选黑龙江产小粒菟丝子雌激素样作用的质量标志物，为后续实验研究及质量控制提供参考依据。方法 采用超高效液相色谱-四极杆飞行时间串联质谱（UPLC-Q-TOFMS/MS）技术对菟丝子提取物进行定性分析，并建立菟丝子不同极性部位的指纹图谱。以小鼠子宫系数、子宫内膜厚度和血清雌激素水平评价不同极性部位的雌激素样活性，通过双变量相关分析和灰色关联度分析构建化学物质组与雌激素效应的组-效相关性，筛选质量标志物，并建立其中5个质量标志物的含量测定方法。结果 在正、负离子扫描模式下共发现10个与菟丝子雌激素样作用相关的指标成分，分别为金丝桃苷、紫云英苷、豆甾醇、新菟丝子苷C、芹菜素、山柰酚、6-O-反式-对香豆酰基-呋喃果糖-（2→1）-吡喃葡萄糖苷、槲皮素、异鼠李素和2，6-十八碳二炔酸。对其中5个质量标志物进行了含量测定，质量分数分别为金丝桃苷（2.753±0.097）mg/g、槲皮素（1.139±0.107）mg/g、芹菜素（1.104±0.047）mg/g、山柰酚（1.144±0.079）mg/g、异鼠李素（0.697±0.074）mg/g。结论 基于组-效关联思路可初步发现黑龙江产小粒菟丝子的质量标志物，所建立的质量标志物含量测定方法准确、稳定。

Objective To preliminarily screen out the estrogen-like quality markers of small grain Cuscuta chinensis from Heilongjiang Province, so as to provide reference for its subsequent experimental research and quality control. Methods UPLC-Q-TOFMS/MS was used to qualitatively analyze the extract of C. chinensis and the fingerprints of different polar fractions were established. The estrogenic activity of different polar fractions was evaluated with uterine coefficient, endometrial thickness and serum estrogen level of mice. The bivariate correlation analysis and gray relational analysis were used to construct the composition-activity relationship between chemicals and the effects of estrogen for screening quality markers. And the content determination methods of the five quality markers were established. Results A total of 10 quality markers related to the estrogenic effect from C. chinensis were found in the positive and negative ion scanning modes. They were hyperoside, astragalin, stigmasterol, neocuscutoside C, apigenin, kaempferol, 6-O-(E)-p-coumaroyl-β-D-fructofuranosyl-(2→1)-α-D-glucopyranoside, quercetin, isorhamnetin, and 2,6-octadecadiynoic acid. The contents of the five quality markers were determined as follows:hyperoside (2.753±0.097) mg/g, quercetin (1.139±0.107) mg/g, apigenin (1.104±0.047) mg/g, kaempferol (1.144±0.079) mg/g and isorhamnetin (0.697±0.074) mg/g. Conclusion The quality markers of small grain C. chinensis from Heilongjiang Province can be screened out according to the composition-activity relationship, and the method for the detect the concentrations of the quality markers is accurate and stable.

R285.5

黑龙江省普通本科高等学校青年创新人才培养计划项目（UNPYSCT-2018135）；哈尔滨商业大学博士科研启动基金（2019DS115）