目的 建立UPLC-MS/MS方法同时测定木蝴蝶中木蝴蝶苷A、木蝴蝶苷B、黄芩苷、黄芩素、白杨素、高车前素、野黄芩素、金丝桃苷、芹菜素、槲皮素10种主要成分。方法 采用负离子多反应监测（MRM）模式，ESI离子源，Kinetex C₁₈色谱柱（100 mm×3.0 mm，2.6 μm）进行分离，流动相为乙腈-甲醇-0.5%甲酸水，梯度洗脱，分析时间为7 min。结果 所测10种主要成分在测定浓度范围内线性关系良好，相关系数均大于0.996 6；精密度、重复性和稳定性良好；平均加样回收率为94.0%～104.5%，RSD≤3.21%。结论 首次建立可同时测定木蝴蝶中10种主要成分的UPLC-MS/MS方法，该法操作简便，灵敏度高，专属性好，分析速度快，可用于中药材木蝴蝶的质量控制。

Objective To develop and validate an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of ten component (baicalin-7-glucoside, oroxin BCAS, baicalin, baicalein, chrysin, hispidulin, scutellarein, hyperoside, apigenin and quercetin) in Oroxylum indicum. Methods The ten components were separated within 7.0 min on a Kinetex C₁₈ column (100 mm×3.0 mm, 2 μm) using a mobile phase consisted of methanol, acetonitrile and 0. 1% formic acid water solution with gradient elution. Results All of the analytes showed good linearity (r ≥ 0. 996 6) in the tested ranges. The precision, repeatability and stability of the method were good for the ten components. The average recoveries were in the range of 94.0%-104.5% with relative standard deviations (RSD) ≤ 3.21%. Conclusion A new rapid and reliable UPLC-MS/MS method was developed and validated to evaluate the quality of O. indicum for the first time.

R286.2

河北省卫计委课题（20170287）；河北省高等学校科学技术研究重点项目（ZD2019054）；河北省自然科学基金面上项目（H2019206219）