目的 研究白芍药材不同加工方法与质量之间的相关性，筛选最佳的加工方法。方法 从白芍的道地产区采集芍药鲜根，针对白芍药材加工过程中去皮、蒸煮、增白等关键环节，设计了7种不同加工方法加工白芍。用测色仪测定白芍表面的颜色，用UPLC测定白芍中芍药苷、芍药内酯苷、苯甲酰芍药苷、氧化芍药苷、没食子酸、儿茶素和1，2，3，4，6-五没食子酰葡萄糖的含量。并对白芍颜色与药效成分含量之间进行相关性分析，综合评价白芍的质量和加工方法。结果 不同方法加工白芍的颜色和药效成分的含量不同。以芍药鲜根先煮八分熟、去皮，再煮透心、干燥的白芍颜色最白（L^*值为72.64）；以芍药鲜根先去皮，后蒸透心，再干燥的白芍的芍药苷质量分数最高（50.08 mg/g）；以芍药鲜根先煮透心，后去皮，再干燥的白芍药效成分的总量最高（71.49 mg/g）。结论 白芍的白色程度与药效成分含量之间略呈负相关。白芍的颜色白与药效成分含量高难于兼顾。可根据对白芍外观颜色和内在质量的要求，选用不同的加工方法。

Objective To investigate the correlation between the various processing procedures and quality of Paeoniae Radix Alba (PRA) to optimize its processing method. Methods The fresh roots of Paeonia lactiflora were harvested at its main cultivating area. Then, a total of seven kinds of processing methods were designed according to the key processing steps, i.e. peeling, cooking or steaming, whitening, etc. The fresh roots of P. lactiflora were processed into PRA by those various processing methods. The surface colors of these PRA samples were measured by colorimeter. Meanwhile, seven bioactive compounds including paeoniflorin, albiflorin, benzoylpaeoniflorin, oxypaeoniflorin, gallic acid, catechin and 1,2,3,4,6-pentagalloylglucose in PRA samples were quantified by ultra-high performance liquid chromatography. PRA qualities and processing methods were comprehensively assessed by correlation analysis between color value and content of bioactive compounds in PRA. Results Both the values of surface color and bioactive compounds were significantly varied among PRA samples processed by various processing methods. The surface color was the whitest with L* value of 72.64 for the PRA sample processed by the procedure that the fresh root was firstly boiled medium well, then peeled, boiled well and dried. The highest content of paeoniflorin (50.08 mg/g) in PRA processed by the procedure that the fresh root was peeled, then steamed and dried. In addition, the highest level of total amount for the seven bioactive compounds (71.49 mg/g) was found in PRA processed by the procedure that the fresh peony root was firstly cooked well, then peeled and dried. Conclusion The correlation is slightly negative between the whiteness of surface color and the content of bioactive compounds in PRA. It is difficult to obtain PRA with both whiteness and high level of bioactive compounds. The various processing methods are suggested for the different requirements such as white surface color or high level of bioactive compounds in PRA.

R286.2

四川省高校科研创新团队建设计划（18TD0017）；四川省教育厅资助科研项目（16ZA0113）；成都中医药大学杏林学者学科人才科研提升计划（CXTD2018018）