目的 对3个产地紫苏叶药材特征性成分进行差异性分析。方法 采集了不同主产区20批紫苏叶药材，分别采用指纹图谱、UFLC-Q-TOF-MS定性鉴别及HPLC多指标成分定量结合聚类分析的方法对紫苏叶药材的特征性组分进行差异性分析。结果 3个产区20批次药材叶指纹图谱相似度均在0.900以上，因而无法通过相似度区分不同产地药材；进一步以UFLC-Q-TOF-MS及对照品对照鉴定了指纹图谱中21个特征峰成分；并对其中6个黄酮苷、2个酚酸及1个单萜主要特征组分含量进行了对比分析，结果显示总黄酮苷含量3个产地差异并不明显，而酚酸及紫苏醛成分产地间差异显著，其中安徽太和县产区药材中酚酸总含量明显高于广东英德和河北安国产地，广东英德产紫苏叶中紫苏醛成分含量较高，结合聚类分析可以将3个产地紫苏叶药材进行区分。结论 多指标成分定性定量能较准确地分析不同产地紫苏叶药材的质量状况，比单一含量测定或指纹图谱相似度评价方法更为客观，也为紫苏叶药材质量控制及产地优选提供了参考。

Objective To comparatively analyze the characteristic components of Perillae Folium from different origins. Methods Twenty batches of Perillae Folium were obtained from three geographical regions. Comparative study on the characteristic components of Perillae Folium from three geographical origins was conducted by Cluster analysis combined with UFLC-Q-TOF-MS and muti-component quantification. Results The similarity of fingerprints of the 20 batches of herbs was above 0. 900, so it was hard to distinguish the different origins of herbs by similarity. Then a total of 21 characteristic peak components were identified by UFLC-Q-TOF-MS analysis coupled with standard compounds information. The content of the main components of six flavonoid glycosides, two phenolic acids and one terpenoid was determinated by HPLC. The results showed that there was no significant difference in the content of flavonoid glycosides among the three regions. However, the difference in the content of phenolic acids and perilladehyde was significant. The content of two phenolic acids which came from Anhui Taihe's samples was significantly higher than those from Guangdong Yingde and Hebei Anguo. In addition, the content of perilladehyde of Guangdong Yingde was obviously higher than the others. And the cluster analysis could be used to distinguish three geographical regions of Perillae Folium. Conclusion Qualitative and quantitative analysis of multi-index components could obtain the contents of ingredients and the quality of Perillae Folium from different regions, which provides a reference for the quality control and preferred origin of Perillae Folium.

R286.2

江苏省中药资源产业化过程协同创新中心课题（ZDXM-2-8）；江苏省“六大人才高峰”项目（2010-YY-009）；江苏高校优势学科建设工程项目（ysxk-2014）；江苏高校品牌专业建设工程项目（PPZY2015A070）