目的 观察益气化湿通络方对慢性肾功能衰竭（CRF）大鼠炎症及纤维化的改善作用，并探讨其可能的分子机制。方法 用Platt法制备CRF大鼠模型，将造模成功的24只SD雄性大鼠分为模型组、益气化湿通络方组和贝那普利组，每组8只，同时设假手术组（8只）。观察大鼠血生化指标血肌酐（Scr）、尿素氮（BUN）及通过HE和天狼星红染色观察残肾组织病理改变；通过实时荧光定量PCR检测FN mRNA表达水平；Western blotting技术观察大鼠肾组织核转录因子-κB（NF-κB）、细胞间黏附分子-1（ICAM-1）、转化生长因子-β1（TGF-β1）、纤维连接蛋白（FN）蛋白表达情况；免疫组化法观察FN蛋白表达情况，免疫荧光法观察NF-κB蛋白的表达情况。结果 模型组大鼠Scr、BUN水平升高，病理改变呈现明显的炎症浸润及胶原沉积，NF-κB、ICAM-1、TGF-β1、FN蛋白表达水平增高。经益气化湿通络方及贝那普利干预后Scr、BUN值降低，炎症减少，胶原沉积减少，NF-κB、ICAM-1、TGF-β1、FN蛋白表达减少。结论 益气化湿通络方改善了CRF模型大鼠的肾功能，通过调节NF-κB通路和TGF-β1蛋白水平减缓残存肾脏炎症及纤维化的病理进程。

Objective To observe the ameliorative effect of Yiqi Huashi Tongluo Formula on inflammation and fibrosis in rats with chronic renal failure and explore its possible related mechanism. Methods The rat model of chronic renal failure was established by Platt method. 24 SD male rats were divided into model group (Model), Yiqi Huashi Tongluo Formula group (YHT) and benazepril group (Benazepril Hydrochloride, BH) with eight rats in each group. At the same time, eight sham-operated control groups were set up with (Sham). The blood biochemical indexes Scr and BUN were observed, and the pathology of remnant kidney was observed by HE and Sirius red staining. The expression of FN mRNA protein was detected by real-time fluorescence quantitative PCR, and the expression of NF-κB (activated nuclear factor-kappaB), ICAM-1 (intercellular adhesion molecule-1), TGF-β1 (transforming growth factor-β1) and FN (fibronectin) in rat kidney tissue was observed by Western blot technique, and the expression of FN protein was observed by immunohistochemistry and NF-κB protein was observed by immunofluorescence staining. Results In the model group, the levels of Scr and BUN were increased, the pathological film showed obvious inflammatory infiltration and collagen deposition, and the expression levels of NF-kappaB, ICAM-1, TGF-β1 and FN were increased. After the intervention of Yiqi Huashi Tongluo Formula or benazepril, the values of Scr and bun were decreased, inflammation level was reduced, collagen deposition was decreased, and the expression levels of NF-κB, ICAM-1, TGF-β1 and FN protein were decreased. Conclusion Yiqi Huashi Tongluo Formula can improve renal function, reduce inflammation and inhibit fibrosis in rats with 5/6 renal resection, and its mechanism is related to affecting NF-κB signaling pathway and regulating TGF-β1 protein. It is suggested that Yiqi Huashi Tongluo Formula can effectively treat chronic renal failure.

R285.5

国家自然科学基金资助项目（81373804）；河北省科技计划项目（14277708D）；河北省研究生创新资助项目（XCXZZBS2019003）