目的 确定指标权重系数，建立乌甘袋泡茶饮片最佳提取工艺并进行抗炎机制研究。方法 比较层次分析法（AHP）、基于指标相关性的权重确定方法（CRITIC）、AHP-CRITIC混合加权法确定权重系数，对正交试验结果进行综合得分比较，优选乌甘袋泡茶饮片提取工艺。通过HE染色观察大鼠咽部黏膜病理变化，测定血清中白细胞介素-1β（IL-1β）、IL-6β和肿瘤坏死因子-α（TNF-α）含量变化探讨抗炎作用。结果 AHP-CRITIC混合加权法科学优选出最佳煎煮工艺参数为饮片浸泡30 min，第1次加7倍量水煎煮45 min；第2、3次加6倍量水煎煮30 min，浓缩至饮片质量5倍量。乌甘袋泡茶高、中剂量组可以改善咽部黏膜组织病变，可以降低L-1β、IL-6β、TNF-α含量。结论 通过AHP-CRITIC混合加权法正交试验优选的乌甘袋泡茶饮片提取工艺稳定。此工艺条件下的饮片提取液继续浓缩制备的乌甘袋泡茶对大鼠慢性咽炎有一定的治疗作用，通过降低炎症因子发挥药效。

Objective To determine the index weight coefficient, establish the best extraction process of Wugan Tea, and study the anti-inflammatory mechanism. Methods Compared with analytic hierarchy process (AHP), based on criteria importance through intercriteria correlation (CRITIC) and AHP-CRITIC mixed weighting method to determine weight coefficient, comprehensive score of orthogonal test results was compared to optimize the extraction process of Wugan Tea. The pathological changes of pharyngeal mucosa in rats were observed by HE staining. The changes of serum interleukin-1 (IL)-1β, IL-6β, and tumor necrosis factor (TNF-α) levels were observed to explore the anti-inflammatory effects. Results The best decoction process parameters were optimized by Mixed weighted AHP-CRITIC method as following:soaking pieces for 30 min, adding seven times of water to boil for 45 min for the first time, adding six times of water to boil for 30 min for the second and third times, and concentrating to five times of pieces mass. The high and medium dose of Wugan Tea can improve the pathological changes of pharyngeal mucosa and reduce the content of L-1β, IL-6βand TNF-α. Conclusion The extraction process of the tea pieces from Wugan Tea optimized by AHP-CRITIC mixed weighted orthogonal test is stable. The concentrated and prepared pieces has a certain therapeutic effect on chronic pharyngitis in rats, and exerts its efficacy by reducing inflammatory factors.

R284.2

中藏药大健康产品开发创新团队（2018C031）；川产道地药材（乌梅）综合开发与区域发展项目（2017ZY006）