目的 研究鹿藿Rhynchosia volubilis的化学成分。方法 采用硅胶、ODS、Toyopearl HW-40C、Sephadex LH-20柱色谱以及半制备HPLC等方法进行纯化，根据理化性质及波谱数据对化合物进行结构鉴定。结果 从鹿藿石油醚提取物中分离得到13个化合物，分别鉴定为（-）-sigmoidin E（1）、lupinifolin（2）、precatorin B（3）、木豆酮（4）、槐花多糖B（5）、5，3'-二羟基-4'-甲氧基-5'-γ，γ-二甲基烯丙基-2″，2″-二甲基吡喃并[5，6：6，7]异黄烷酮（6）、染料木素（7）、甘草异黄酮A（8）、erylatissin B（9）、neo-bavaisoflavone（10）、羽扇豆醇（11）、桦木醛（12）、穿贝海绵甾醇（13）。化合物1～10均为含异戊烯基的黄酮类，其中化合物1、2为二氢黄酮、3～6为二氢异黄酮、7～10为异黄酮。化合物11～12为羽扇豆烷型三萜，化合物13为甾醇类。结论 化合物1、5～6、8～10、12、13均为首次从鹿藿属植物中分离得到，化合物1～3、5～13均为首次从该植物中分离得到。

Objective To study the chemical constituents from Rhynchosia volubilis. Methods The compounds were isolated and purified by a combination of various chromatographic techniques including silica gel, ODS, Toyopearl HW-40C, Sephadex LH-20, and semi-preparative HPLC chromatography. Their structures were identified by physicochemical properties and spectroscopic data. Results Thirteen compounds were isolated from the petroleum ether extracts of R. volubilis and their structures were elucidated as (-)-sigmoidin E (1), lupinifolin (2), precatorin B (3), cajanone (4), sophoraisoflavanone B (5), 5,3'-dihydroxy-4'-methoxy-5'- γ,γ-dimethylallyl-2″,2″-dimethylpyrano[5,6:6,7] isoflavanone (6), genistein (7), licoisoflavone A (8), erylatissin B (9), neo-bavaisoflavone (10), lupeol (11), betulinic aldehyde (12), and clionasterol (13). Compounds 1-10 were all prenylated flavonoids, of which compounds 1-2 were dihydroflavones, compounds 3-6 were dihydroisoflavones, and compounds 7-10 were isoflavones. Compounds 11-12 were lupine triterpenoids, and compounds 13 was a sterol. Conclusion Compounds 1, 5-6, 8-10, 12 and 13 are isolated from the genus for the first time, while compounds 1-3 and 5-13 are separated from this plant for the first time.

R284.1

国家重点研发计划——“生殖健康及重大出生缺陷防控研究”重大专项资助项目（2016YFC1000905）