[关键词]
[摘要]
目的 关联分析红花质量标志物(Q-marker)羟基红花黄色素A的含量、抗凝血及抗氧化结果,建立一种基于Logistic回归分析的红花饮片等级评价方法。方法 采用超高效液相色谱(UPLC)法测定红花Q-marker羟基红花黄色素A的含量,以凝血酶时间(TT)反映红花的抗凝血活性,以清除羟自由基及DPPH自由基的能力反映红花体外抗氧化活性,运用Logistic回归分析法将含量指标和生物活性指标进行关联分析,最终建立用于红花饮片等级评价的Logistic回归模型。结果 建立的Logistic模型具有良好的稳定性及等级预测能力(训练集及测试集样本的P值均大于0.999),将19批红花分为了优、良、中、差4个等级。结论 基于含量测定及生物活性建立的Logistic模型可以用来评价红花饮片质量优劣,为其质量控制提供一定的参考。
[Key word]
[Abstract]
Objective To establish a Logistic model for quality assessment of Carthamus tinctorius based on content determination and bioactivity. Methods A method to determinate hydroxy safflor yellow A by using ultra-high performance liquid chromatography (UPLC) was proposed. The activity of anticoagulant was reflected by thrombin time (TT) and the anti-oxidant activity was expressed by scavenging ability of hydroxyl radical and DPPH radical. Later, a Logistic model grade evaluation of C. tinctorius was constructed based on correlation analysis between content and biological activity Logistic.Results The constructed Logistic model had outstanding stability and high prediction accuracy of grades and it divides 19 batches of C. tinctorius into four grades. Conclusion The Logistic model based on content determination and biological activity is applicable to assess quality of C. tinctorius slices and provides some reference for quality control.
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[基金项目]
国家自然科学基金资助项目(81501229);国家自然科学基金资助项目(81773919);国家自然科学基金资助项目(81503491);国家中药标准化项目(ZYBZH-C-QIN-45);陕西省科技统筹创新工程计划项目(2016KTTSSF01-06-01);陕西省创新人才推进计划青年科技新星项目(2017KJXX-71)