目的 建立高效液相紫外荧光（HPLC-UV-FLD）衍生系统，利用显微注射荧光探针技术对恭城油茶提取物中抗氧化活性成分进行初步评价。方法 采用过氧化氢急性损伤实验及羟自由基清除能力实验对恭城油茶的抗氧化活性进行检测，利用HPLC-UV-FLD衍生系统对其中的抗氧化活性成分进行筛选，并采用超高压液相色谱-四级杆-飞行时间质谱技术（UPLC-Q/TOF-MS）对收集得到的活性成分峰进行定性分析。同时通过显微注射过氧化氢荧光探针分子（NBCD）检测活体果蝇体内过氧化氢水平，探究恭城油茶及其中筛选出的活性成分在体内清除过氧化氢的作用。结果 该方法方便快速，在恭城油茶提取物中筛选出了表没食子儿茶素（EGC）、表没食子儿茶素没食子酸酯（EGCG）和表儿茶素没食子酸酯（ECG）3种主要的抗氧化活性成分。同时检测出恭城油茶及其抗氧化活性成分能够显著降低果蝇体内过氧化氢水平。结论 为进一步研究恭城油茶抗氧化提供物质基础，并且为天然产物中的抗氧化活性成分的筛选方法提供参考。

Objective A high performance liquid chromatography-ultraviolet-fluorescence (HPLC-UV-FLD) derivative system was established and the anti-oxidant activity of the extract of Gongcheng Tea was preliminarily evaluated by using a micro-injector imaging system combined with a fluorescent probe. Methods Hydrogen peroxide assay and hydroxyl radical scavenging ability experiments were carried out on the anti-oxidant activity of Gongcheng Tea. The anti-oxidant components of Gongcheng Tea were screened by HPLC-UV-FLD post column derivation system and analyzed by an ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF MS). At the same time, the hydrogen peroxide scavenging activity of tea and active components were tested by a micro-injector imaging system combined with a hydrogen peroxide fluorescent probe (NBCD) in living Drosophila melanogaster. Results The method was simple and rapid, and three main anti-oxidant compounds, epigallocatechin, epigallocatechin gallate, and epicatechin gallate were found in Gongcheng Tea. And it was found that they could significantly reduce the level of hydrogen peroxide in D. melanogaster. Conclusion It laid a material basis for the further study of the anti-oxidant activity of Gongcheng Tea, and provided a reference for the screening of anti-oxidants in natural products.

广西科技计划项目：恭城油茶系列国际化健康产品开发研究（桂科AD17129010）