目的 采用Box-Behnken设计-响应面法（BBD-RSM）优选木鳖子霜的最佳炮制工艺。方法 以丝石竹皂苷元3-O-β-D-葡萄糖醛酸甲酯、β-谷甾醇、齐墩果酸含量及脂肪油含量的指标归一值（OD）为综合评价指标，采用3因素3水平的BBD-RSM设计试验，考察烘制时间、烘制温度、压制时间对木鳖子霜炮制工艺的影响。结果 优化得最佳工艺条件：烘制时间1.68 h、烘制温度80℃、压制时间30 min，OD值为0.777。考虑实际情况，对烘制时间进行微调得出木鳖子最佳炮制工艺，即烘制时间为1.7 h、烘制温度为80℃、压制时间为30 min。并通过所得条件平行制备3批样品，计算得OD值分别为0.779、0.783、0.766，其RSD为1.15%。结论 采用BBD-RSM优选的炮制工艺稳定，模型预测效果良好，建立了木鳖子霜炮制新工艺。

Objective To optimize the optimum processing technology of Momordicae Semen cream using Box-Behnken design-response surface method (BBD-RSM). Methods The overall desirability (OD) of indexes of the content of 3-O-β-D-glucuronic acid methyl ester, β-sitosterol, and oleanolic acid and the content of fatty oil in gypsogenin was comprehensively evaluated, and BBD-RSM with three factors and three levels was used to study the effect of baking temperature and time, and pressing time on the processing technology of Momordicae Semen cream. Results According to the experiment, the optimum processing conditions were optimized:the baking time 1.68 h, the baking temperature 80℃, the pressing time 30 min, and the OD value was 0.777. Considering the actual situation, the optimum processing technology of Momordicae Semen was obtained by fine-tuning the baking time (A). That was, the baking time was 1.7 h, the baking temperature was 80℃, and the pressing time was 30 min. Also, the calculated OD values were 0.779, 0.783, 0.766, and its RSD was 1.15% through the obtained conditions in parallel with three batches of samples. Conclusion The processing technology optimized by Box-Behnken design-response surface method has the advantages of stable, good model prediction effect and a new processing technology for Momordicae Semen cream production.

安徽中医药大学校级探索科研项目（2016ts041）；2015年中医药部门公共卫生专项资金“中药炮制技术传承基地建设”项目