目的 应用响应曲面分析法阐明当归-红花药对活血效应相互作用的范围、性质与程度。方法 采用冰水浴与注射盐酸肾上腺素复制大鼠急性血瘀模型，给予不同配比（1：0、4：1、2：1、3：2、1：1、2：3、1：2、1：4、0：1）不同剂量当归-红花药对后，观察其对血液流变学各指标的影响，采用多指标综合指数法对各指标进行整合，然后运用响应曲面分析法对整合效应结果进行分析，应用非线性回归的方法确定量效曲线的各个参数，建立三维响应曲面模型，应用Matlab软件构建响应曲面的三维图形。结果 发现当归-红花药对配比在0.8：1～1.1：1以及在当归剂量为0～5 g，红花8～11 g这个区域之间表现为协同作用（相互作用值：-0.2～-0.8）；在当归剂量为0～0.2 g，红花为0.2～1 g表现为拮抗作用，但拮抗作用不明显，相互作用值为0.2左右，而其他比例并未表现出明显的协同或拮抗作用。结论 采用响应曲面分析法可定量研究中药配伍药物相互作用，为药对相关研究提供了思路与方法的参考。

Objective To demonstrate the range, properties and extent of the interaction for activating blood effects in herb pair containing Angelicae Sinensis Radix and Carthami Flos (ASR-RC) by response surface method. Methods Based on the acute blood stasis rats model, the hemorheological indexes of ASR-RC with different proportions (1:0, 4:1, 2:1, 3:2, 1:1, 2:3, 1:2, 1:4, 0:1) and different concentration were observed. Then all data were integrated to the total activating blood circulation effect value by comprehensive index method. The interaction was analyzed by response surface method. Model parameters were estimated with nonlinear regression. The three-dimensional response surfaces were constructed with Matlab software. Results The proportion from 0.8:1 to 1.1:1 of ASR-RC had synergetic effects, and when the dose of Angelicae Sinensis Radix ranged from 0 g to 5 g, and Carthami Flos from 8 g to 11 g, the herb pair also had synergetic effects (with the value between -0.2 and -0.8). In addition, when the dose of Angelicae Sinensis Radix ranged from 0 g to 0.2 g, and Carthami Flos ranged from 0.2 g to 1 g, the herb pair had antagonism action (with the value of 0.2). Conclusion The research results could provide scientific evidence for clinical application of ASR-RC. And quantitative analysis on drug interactions of herb pair by response surface method could also provide references for relevant studies.

国家自然科学基金资助项目（81274058，81573714）