目的 运用红外光谱法和二维相关光谱技术对白芍、赤芍及其醇提物所含化学成分的红外谱图整体变化规律进行解析和鉴别。方法 采用傅里叶变换红外光谱法和二维相关光谱分析技术，对白芍和赤芍及其醇提物进行鉴别分析。结果 白芍、赤芍原药材一维红外光谱显现了草酸钙特征峰和位于950~1 200 cm^-1处的淀粉阶梯峰，但峰形、峰位稍有差异；二阶导数处理后，二者在518/517、989 cm^-1等处的特征峰得以显现，另外，赤芍在989、1 015、1 052、1 078、1 105、1 161 cm^-1的峰强度均高于白芍，且峰形与白芍也不尽相同，这与两者所含淀粉的量和结构差异有关。白芍、赤芍醇提物一维光谱中都有芍药苷的特征峰（1 716、1 451、1 347、1 277、714 cm^-1）；图谱经二阶导数处理后可以看出两者的峰形、峰位相差较大，这与两者的糖（苷）类量和结构存在差异有关；在二维相关红外光谱中，两者在887、968、1 008、1 190、1 305 cm^-1处均有糖（苷）类化合物的自动峰，不同的是白芍在1 190 cm^-1处的自动峰强度最大，赤芍在968 cm^-1处的自动峰强度最大，进一步佐证了两者所含糖（苷）类化合物有差异。结论 红外光谱法和二维相关光谱技术提供了大量白芍、赤芍的整体结构信息，递进式地验证了两者所含物质结构和量的差异，可以初步地鉴定白芍和赤芍，为今后系统完整的鉴定工作打下基础。

Objective The Fourier transform infrared spectroscopy (FT-IR) and two-dimension IR correlation infrared spectroscopy (2D-IR) were used for the identification of Paeoniae Radix Alba and Paeoniae Radix Rubraand their alcohol extracts. Methods The FT-IR spectra method and 2D-IR correlation spectra method were used. Results The structural information of samples indicated that Paeoniae Radix Alba and Paeoniae Radix Rubra contained a large amount of calcium oxalate and starch, since some characteristic absorption peaks of the calcium oxalate could be observed; And some characteristic absorption peaks in the range of 950-1 200 cm^-1 of the starch can be observed, but their shape and location revealed minor differences. In the secondary derivative infrared spectra (SD-IR), both Paeoniae Radix Alba and Paeoniae Radix Rubra can be observed with the characteristic absorption peaks which in 518/517, 989 cm^-1 and so on. Besides, these peaks at 989, 1 015, 1 052, 1 078, 1 105, and 1 161 cm^-1 of Paeoniae Radix Rubra, almost of them which are single peak, were stronger than Paeoniae Radix Alba's and the same peaks in Paeoniae Radix Alba were jagged peaks, showing that the difference were related to the contents and structure of starch in Paeoniae Radix Alba and Paeoniae Radix Rubra. The characteristic absorption peaks of the peoniflorin which arouse at 1 716, 1 451, 1 347, 1 277, and 714 cm^-1 in the FT-IR spectra of their alcohol extracts can be found. Moreover, the shape and intensity of the peaks were more distinct in the secondary derivative IR spectra of the different parts. For example, in the range of 900-980 cm^-1, Paeoniae Radix Alba presented two groups of peaks: 935, 919 cm^-1 (strong) and 962, 949 cm^-1 (weak), while Paeoniae Radix Rubra only presented one group of peaks:941, 920 cm^-1 (middle), showing that the difference was related to the content and structure of glucoside in Paeoniae Radix Alba and Paeoniae Radix Rubra. In the 2D-IR spectra, both had five auto-peaks at 887, 968, 1 008, 1 190, and 1 305 cm^-1, which were the auto-peaks of glucoside, but the strongest auto-peak of Paeoniae Radix Alba was at 1 190 cm^-1 and that of Paeoniae Radix Rubra's was at 968 cm^-1. The spectra testified the glucoside compounds in Paeoniae Radix Alba and Paeoniae Radix Rubra were different. Conclusion A lot of information of Paeoniae Radix Alba and Paeoniae Radix Rubra can be provided by FT-IR spectra method and 2D-IR correlation spectra method which can testify that the content and structure of substance in Paeoniae Radix Alba and Paeoniae Radix Rubra were different and can be used to analyze and distinguish Paeoniae Radix Alba and Paeoniae Radix Rubra preliminarily which can make a good foundation for further research.

国家自然科学基金资助项目（81303217）