目的 通过电子克隆技术对百脉根细胞亲环素（cyclophilin）基因进行预测。方法 以大豆cyclophilin序列为探针，基于NCBI中百脉根的EST数据库和CAP3在线软件进行序列拼接，利用生物信息学数据库及相关软件对其结构和功能进行预测分析。结果 百脉根cyclophilin基因全长1 346 bp，包含771 bp的开放阅读框，编码256个氨基酸，该蛋白为亲水性蛋白。结论 为进一步解释基因的分子功能奠定理论及实验基础。

Objective Using electronic cloning technology to predict cyclophilin gene of Lotus corniculatus. Methods Using glycine max cyclophilin sequence as probe sequence, based on EST sequence from NCBI and assembled by CAP3 sequence assembly programme, using bioinformatic database and related software, the structure prediction and function analysis were performed. Results The full length of cyclophilin gene was 1 346 bp, it contained a 771 bp ORF, encoding 256 amino acids, and the protein was a hydrophilic protein. Conclusion The study is intended to further explain the molecular genetic function theory and experimental basis.

国家自然基金资助项目（81274010）；黑龙江省杰出青年基金（JC201101）；黑龙江中医药大学“优秀创新人才支持计划”（2012001）；哈尔滨市优秀学科带头人基金（2014RFXXJ122）；黑龙江省教育厅科学技术研究项目（12541743）