目的 建立了飞龙掌血Toddalia asiatica根皮、茎皮高效液相色谱法指纹图谱，并运用相似度评价软件对指纹图谱进行相似度对比，从而为促进飞龙掌血综合利用提供理论依据。方法 采用乙腈-0.1%磷酸水溶液梯度系统，C₁₈柱（200 mm×4.6 mm，5 μm）色谱柱，测定波长269 nm，体积流量1.0 mL/min。通过对10批不同产地不同季节采收的飞龙掌血根皮与10批不同产地不同季节的茎皮对比研究，从而建立飞龙掌血的指纹图谱，并计算相似度。结果 不同产地及不同季节采集的飞龙掌血根皮与茎皮共有峰的相对保留时间相对一致，但相对峰面积差异较大，说明产地和采收季节的不同会导致相关物质活性成分的量有一定的差异。结论 飞龙掌血根皮与茎皮指纹图谱相似度大于0.9，其组成高度相似。

Objective To promote the comprehensive utilization in the root barks and stem barks of Toddalia asiatica and to establish an HPLC method for the determination of the fingerprints for the root barks and stem barks of T. asiatica. Meanwhile, the similarity was compared by similarity evaluation software based on the fingerprints. Methods HPLC analysis was performed on C₁₈ (250 mm × 4.6 mm, 5 μm) with acetonitrile and 0.1% aqueous solution of phosphoric acid as mobile phase in gradient mode at the wavelength of 269 nm, and the flow rate was 1 mL/min. The similarity was calculated by comparison on the fingerprints derived from 10 bathes of T. asiatica root barks and 10 bathes of T. asiatica stem barks, all the samples were collected from the different areas in different seasons. Results The mutualmode of HPLC fingerprints was set up and the similar degrees to the crude drugs of different sources were compared. The content ratio of active ingredients is different from samples in different areas and seasons. Conclusion The fingerprint similarity is greater than 0.9 between T. asiatica root barks and T. asiatica stem barks, so the compositions are highly similar.

贵州省科技厅项目[黔科合SY字（2015）3031]