目的 对江西地方山药（瑞昌山药、南城淮山、永丰淮山和广丰千金薯）微型块茎萌发苗耐盐胁迫的生理调节机制进行研究，比较江西4个地方山药微型块茎萌发苗的耐盐性，为瑞昌山药、南城淮山、永丰淮山和广丰千金薯的耐盐机制研究与耐盐育种提供参考。方法 采用分光光度法探究盐胁迫下江西地方山药微型块茎萌发苗部分生理生化指标的变化，同时利用模糊数学隶属函数公式和SPSS 19.0软件对江西地方山药微型块茎萌发苗的耐盐性进行隶属函数及主成分分析和聚类分析。结果 在0~300 mmol/L盐胁迫下，对于总叶绿素量和根系活力而言，江西4个山药微型块茎萌发苗的总叶绿素量和根系活力均呈显著下降的趋势。对于可溶性蛋白量而言，瑞昌山药、南城淮山和广丰千金薯微型块茎萌发苗的可溶性蛋白量呈先增后降的趋势，而永丰淮山微型块茎萌发苗的可溶性蛋白量呈显著下降。对于可溶性总糖、脯氨酸（Pro）和丙二醛（MDA）量以及PMP而言，江西4种山药微型块茎萌发苗的可溶性总糖、Pro和MDA量以及PMP均呈显著增加。对于POD、SOD和过氧化氢酶（CAT）活性而言，江西4种山药微型块茎萌发苗的POD、SOD和CAT活性均呈先增后降的趋势。基于隶属函数及SPSS 19.0软件的主成分分析，盐胁迫下江西地方山药微型块茎萌发苗10个生理生化指标被归纳成3个主成分，江西地方山药微型块茎萌发苗的耐盐性从强到弱依次为广丰千金薯 > 永丰淮山 > 瑞昌山药 > 南城淮山。通过SPSS 19.0软件的聚类分析，江西4种地方山药的耐盐性分成2种类型，广丰千金薯和永丰淮山为耐盐较强的品种，而南城淮山和瑞昌山药为耐盐较弱的品种。结论 揭示了江西4种地方山药微型块茎萌发苗耐盐胁迫的生理调节机制，同时对江西4种地方山药的耐盐性进行了客观评价，这些结果将会为江西4种地方山药微型块茎的大田播种提供理论依据。

Objective The physiological regulation mechanism of seedlings germinated from Jiangxi local yam (Ruichang yam, Nancheng yam, Yongfeng yam, and Guangfeng yam) microtubers under salt stress was studied and the salt tolerance of seedlings germinated from microtubers of four kinds of local yams in Jiangxi was compared, which provided some references for salt tolerance mechanism research and salt tolerance breeding of four local yams in Jiangxi. Methods Some physiological and biochemical index detection of seedlings germinated from Jiangxi local yam microtubers under salt stress were studied by spectrophotometer method. The subordinate function, principal component analysis (PCA), and clustering analysis of their salt tolerance were accomplished by fuzzy mathematics subordinate function formula and SPSS 19.0 software, respectively. Results The total chlorophyll content and root activity of seedlings germinated from Jiangxi local yam microtubers under 0—300 mmol/L salt stress decreased significantly. The soluble protein content of seedlings germinated from Ruichang yam, Nancheng yam, and Guangfeng yam microtubers under 0—300 mmol/L salt stress increased firstly and then decreased, while the soluble protein content of seedlings germinated from Yongfeng yam microtubers under 0—300 mmol/L salt stress decreased significantly. The total soluble sugar content, proline content, MDA content and membrane permeability of seedlings germinated from Jiangxi local yam microtubers under 0—300 mmol/L salt stress significantly increased. The POD, SOD, and CAT activities of seedlings germinated from Jiangxi local yam microtubers under 0—300 mmol/L salt stress firstly increased and then decreased. Based on the subordinate function and PCA of SPSS 19.0 software, three principal components of 10 physiological and biochemical indexes of seedlings germinated from Jiangxi local yam microtubers under salt stress were induced. The salt tolerance order of seedlings germinated from Jiangxi local yam microtubers was Guangfeng yam > Yongfeng yam > Ruichang yam > Nancheng yam. Through the cluster analysis by SPSS 19.0 software, the salt resistance of four kinds of Jiangxi local yam is divided into two types, the salt tolerance of Guangfeng yam and Yongfeng yam was strong, and of Ruichang yam and Nancheng yam were sensitive to salt stress. Conclusion The regulation mechanism of salt tolerance of seedlings germinated from four kinds of Jiangxi local yam microtubers is revealed and the objective evaluation of salt tolerance of four kinds of Jiangxi local yam is made in the paper, which will provide a theoretical basis for sowing in the field of four kinds of Jiangxi local yam microtubers.

江西省教育厅2014年度科学技术研究一般项目（GJJ14712）；2015年国家级大学生创新创业训练计划项目（201510416007）