目的 定性分析白芍提取物在大鼠血清及脑组织中的化学成分。方法 大鼠ig给予白芍提取物后，采集血液并分离脑组织部位，采用HPLC-ESI-MSⁿ法检测入血及分布于脑组织中的化学成分。结果 通过相应保留时间和质谱数据，以及与文献资料及对照品比对，在大鼠含药血清中共检测到34个化学成分，其中8个为新代谢产物；在全脑组织中共检测到3个化学成分，其中1个为新代谢产物。结论 建立的HPLC-ESI-MSⁿ方法有助于阐明白芍提取物抗抑郁作用的药效物质基础。

Objective Qualitative analysis of chemical components in Paeoniae Alba Radix extracts (PARE) in rat serum and brain. Methods After ig administration of PARE, rat blood and brain tissues were collected. The chemical components in serum and brain were analyzed by HPLC-ESI-MSⁿ. Results By comparing retention time, MS and MSⁿ spectra with data in the literature and reference standards, a total of 34 compounds including eight new metabolites were identified in serum, and three components including one new metabolite were identified in rat brain. Conclusion This research will contribute to elucidate the pharmacodynamic material basis of antidepressant effect of PARE.

国家自然科学基金资助项目（81173163，81173151）