目的 建立高效液相色谱-串联质谱（HPLC-MS/MS）同时测定参乌健脑胶囊（SJC）中8种成分（葛根素、丹参酮II_A、二苯乙烯苷、芍药苷、人参皂苷Re、人参皂苷Rg₁、毛蕊异黄酮苷、黄芩苷）的定量方法。方法 选用YMC-Triart C₁₈色谱柱，以甲醇-0.2%甲酸水溶液为流动相，梯度洗脱，体积流量为0.6 mL/min，柱温40 ℃。质谱条件：ESI源，采用多反应监测模式（MRM），以定量离子对峰面积进行定量。结果 SJC中葛根素、丹参酮II_A、二苯乙烯苷、芍药苷、人参皂苷Re、人参皂苷Rg₁、毛蕊异黄酮苷、黄芩苷分别在20.31~20 310、25.66~25 660、20.45~20 450、50.79~50 790、50.57~50 570、50.38~50 380、10.32~10 320、25.74~25 740 ng/mL内线性关系良好（r≥0.999 0）；精密度良好，重复性良好，RSD均小于2.0%；在室温条件下24 h内稳定；加样回收率为97.98%~102.48%，RSD小于1.6%。所测8种成分在6批样品中的质量分数依次为葛根素1.843~1.860 mg/g、丹参酮II_A 1.618~1.629 mg/g、二苯乙烯苷2.116~2.129 mg/g、芍药苷2.537~2.547 mg/g、人参皂苷Re 0.034~0.041 mg/g、人参皂苷Rg₁ 0.048~0.055 mg/g、毛蕊异黄酮苷0.551~0.564 mg/g和黄芩苷2.333~2.346 mg/g。结论 该方法快速、简便、重复性好，可用于同时测定SJC中多种成分。

Objective To develop a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the simultaneous determination of eight components (puerarin, tanshinone II_A, stibene glucoside, paeoniflorin, ginsenoside Re, ginsenoside Rg₁, calycosin-7-glucoside, and baicalin) in Shenwu Jiannao Capsules (SJC). Methods Chromatographic column: YMC-Triart C₁₈, mobile phase: methanol-water (including 0.2% formic acid), and gradient elution, flow rate: 0.6 mL/min, column temperature: 40 ℃. MS condition: electrospray ionization (ESI) and multiple reaction monitoring (MRM) were adopted, the detected peak areas of ion pairs were used for quantitative determination. Results There was a good linearity between the absorption peak area and the concentration for puerarin, tanshinone II_A, stibene glucoside, paeoniflorin, ginsenoside Re, ginsenoside Rg₁, calycosin-7- glucoside, and baicalin in the ranges of 20.31—20 310, 25.66—25 660, 20.45—20 450, 50.79—50 790, 50.57—50 570, 50.38—50 380, 10.32—10 320, and 25.74—25 740 ng/mL, respectively (r ≥ 0.999 0). The precision was good and RSD was less than 2.0%. The repeatability was good and RSD was less than 2.0%. The stability was good in 24 h. The average recoveries were ranged from 97.98% to 102.48% (RSD ≤ 1.6%). The contents of puerarin, tanshinone II_A, stibene glucoside, paeoniflorin, ginsenoside Re, ginsenoside Rg₁, calycosin-7-glucoside, and baicalin in six batches of samples were in the ranges of 1.843—1.860, 1.618—1.629, 2.116—2.129, 2.537—2.547, 0.034—0.041, 0.048—0.055, 0.551—0.564, and 2.333—2.346 mg/g, respectively. Conclusion The method is rapid, simple, repeatable, and can be used to determine the multi components in different batches of SJC simultaneously.