目的 通过导向分离法发现姜黄Curcuma longa根茎中1个新化合物，并进行分离鉴定及细胞毒活性研究。方法 运用LC-MS分析技术，结合MCI-Gel小孔树脂、ODS-C₁₈反相柱及RP-HPLC等柱色谱手段分离纯化，根据理化性质和波谱数据鉴定化合物的结构。结果 从姜黄根茎甲醇提取物的低极性流分中分离得到1个姜黄素类新化合物，鉴定为姜黄素-没药烷型倍半萜二聚体化合物3,4'''-epoxy-5'''-C-(1α,2β,3β-bisabola-4,10-diene-9-one)-(2→5''')-curcumin（1），该化合物对HepG2、A549和CT-26肿瘤细胞IC₅₀值分别为4.82、8.81和5.40 μmol/L。结论 化合物1为1个姜黄素类新化合物，命名为姜萜素G；其对肿瘤细胞具有显著的细胞毒活性。

Objective Separation and purification of a new curcuminoid from the rhizome of Curcuma longa by guided separation method. Methods The chemical constituent was separated and purified through various kinds of chromatographic methods, including MCI-Gel, ODS-C₁₈ reverse phase column, and RP-HPLC. The structure was elucidated by physicochemical properties and spectral data. Results A new curcuminoid, 3,4'''-epoxy-5'''-C-(1α,2β,3β-bisabola-4,10-diene-9-one)-(2→5''')-curcumin (1), was isolated from the low polar fraction of methanol extracts from the rhizome of C. longa, named as curcuterpene G. This compound exhibited the inhibitory effect to HepG2, A549, and CT-26 tumor cells with the IC₅₀ values of 4.82, 8.81, and 5.40 μmol/L, respectively. Conclusion A new curcuminoid is isolated from the rhizome of C. longa by guided discovery method, and it exhibits notable cytotoxic activities.

国家自然科学基金资助项目（81302742）；教育部春晖计划项目（13205638）；四川省教育厅重点项目（11ZA005）；中国博士后科学基金（2012M511935）