目的 研究忍冬Lonicera japonica根中的化学成分及其抗炎活性。方法 采用反复碱性硅胶柱色谱、Sephadex LH-20柱色谱、薄层色谱、制备液相色谱等方法进行分离纯化，并通过波谱学分析鉴定其化学结构；采用斑马鱼抗炎模型，测定分离所得部分单体成分的抗炎活性。结果 从忍冬根95%乙醇提取物中分离得到14个化合物，并鉴定其结构为3,13-dihydroxystemodan-2-one（1）、大黄酚（2）、palmarumycin CP₂（3）、β-谷甾醇（4）、豆甾醇（5）、豆甾-4,6,8(14),22-四烯-3-酮（6）、erythrinassinate D（7）、羊毛甾醇（8）、齐墩果酸-3-乙酸酯（9）、原儿茶醛（10）、胡萝卜苷（11）、(E)-3-(3,4-二羟基苯亚甲基)-5-(3,4-二羟基苯)-2(3H)-呋喃酮（12）、lomacarinoside B（13）、(2E,6S)-8-(α-L-arabinopyranosyl-(1″→6')-β-D-glucopyranosyloxy)-2,6-dimethyloct-2-eno-1,2″-lactone（14）。结论 化合物1为新化合物，化合物2、3、6、7~9、13均为首次从该植物中分离得到；化合物9、10在100 μg/mL质量浓度下表现出明显的抗炎活性。

Objective To research the chemical composition from the roots of Lonicera japonica and their anti-inflammatory activities. Methods The compounds were isolated and purified by chromatography on silica gel column, Sephadex LH-20 column, preparative thin-layer, and semi-preparative HPLC, etc. Their structures were identified on the basis of spectroscopic data, and parts of the isolated compounds were tested for their anti-inflammatory activities against zebrafish. Results Fourteen compounds were isolated and elucidated as 3,13-dihydroxystemodan-2-one (1), chrysophanol (2), palmarumycin CP₂ (3), β-sitosterol (4), stigmasterol (5), stigmast-4,6,8(14), 22-tetraen-3-one (6), erythrinassinate D (7), lanosterin (8), 3-O-acetyloleanolic acid (9), protocatechuin aldehyde (10), daucosterol (11), (E)-3-(3,4-dihydroxybenzylidene)-5-(3,4-dihydroxyphenyl)-2(3H)-furanone (12), lomacarinoside B (13), and (2E,6S)-8-(α-L-arabinopyranosyl-(1″→6')-β-D-glucopyranosyloxy)-2,6-dimethyloct-2-eno-1,2″-lactone (14). Conclusion Compound 1 is a new compound, and compounds 2, 3, 6, 7—9, and 13 are isolated from L. japonica for the first time. Compounds 9 and 10 show the significant anti-inflammatory activities at 100 μg/mL.

国家自然科学基金面上项目（81473298）；山东省科技发展项目（2014GSF119031）；山东省科技发展项目（2014GZX219003）