目的 在人参属资源中研究20(R)-25-羟基-原人参三醇 [20(R)-25-OH-PPT],为人参皂苷降血糖作用的创新和研发提供科学依据。方法 色谱柱Agilent C₁₈(250 mm×4.6 mm,5 μm);流动相为甲醇-水(70:30);柱温35 ℃;体积流量1.0 mL/min;ELSD气化室温度87.0 ℃,载气流速2.20 L/min;进样体积10 μL。采用HPLC-ELSD法测定人参属植物不同部位提取物(以丙酮为溶剂,采用超声法提取)中20(R)-25-OH-PPT。结果 20(R)-25-OH-PPT在0.001～0.050 mg/mL线性关系良好,在人参茎叶、人参根、人参果、人参花、西洋参茎叶、西洋参根和三七芦头总皂苷中的量分别为3.74%、2.59%、2.37%、0.40%、0.68%、0.22%和0.39%,回收率为98.54%、97.54%、100.35%、101.46%、97.46%、99.14%和99.38%。结论 20(R)-25-OH-PPT存在于人参属植物多个部位中,可作为人参皂苷降血糖作用开发利用的重要依据。

Objective For making full use of our country's rich ginseng resources to look for 25-OH-PPT\ and provide the scientific basis for the innovation and development of ginsenoside hypoglycemic activity. Methods Using chromatographic column Agilent C₁₈ (250 mm × 4.6 mm, 5 μm); mobile phase was CH₃OH-H₂O (70:30); Column temperature was 35 ℃; Flow rate was 1.0 mL/min; the boiling chamber temperature of ELSD was 87 ℃; Flow rate of carrier gas was 2.20 L/min; injection volume was 10 μL. Using acetone as the solvent and ultrasonic extraction to extract the medicinal materials, then using the HPLC-ELSD method to determine the contents of 20(R)-25-OH-PPT in different parts of plants in Panax ginseng. Results 20(R)-25-OH-PPT had a good linear relationship when the concentration was 0.001—0.05 mg/mL. The 20(R)-25-OH-PPT contents in gingseng cauline leaf, gingseng root, gingseng fruit, gingseng flower, cauline leaf of American ginseng, root of American ginseng, and the basal part of stem of P. notoginseng were 3.74%, 2.59%, 0.40%, 2.37%, 0.68%, 0.22%, and 0.39%. The recovery rates were 98.54%, 97.54%, 100.35%, 101.46%, 97.46%, 99.14%, and 99.38%. Conclusion 25(R)-OH-PPT exists in different parts of P. ginseng, and it can be an important basis for the use and development of ginsenoside with hypoglycemic activity.

“十一五”国家重大新药创制(2009ZX09102-114);中药标准提取物的工业化色谱制备平台建设(2009ZX09301-012-105B)