目的 以麦角甾醇为测定指标,建立马勃Lasiosphaera seu Calvatia质量评价的HPLC方法,对42批不同商品马勃中麦角甾醇的量进行比较研究。方法 运用HPLC对多个不同产地和品种的马勃中麦角甾醇进行测定,色谱条件为BDS HYPERSIL C₁₈色谱柱(250 mm×4.6 mm,5 μm);体积流量1.0 mL/min;检测波长282 nm;柱温30 ℃;进样量20 μL;以乙腈(A)-0.1%甲酸水溶液(B)为流动相进行梯度洗脱:0～15 min,95% A,15～50 min,100% A。结果 建立了马勃中麦角甾醇的提取方法和HPLC测定方法。不同品种和产地的马勃中麦角甾醇范围为0.009 9%～0.150 3%。结论 不同商品马勃中麦角甾醇量差异明显,说明目前市售马勃药材品质不均一。因此,对于马勃药材的质量控制尚需进一步规范化和标准化。

Objective The HPLC method was established for assessment of quality of Lasiosphaera seu Calvatia (LSC) based on the determined indicator of ergosterol. The contents of ergosterol in 42 batches of LSC were comparatively analyzed. Methods The contents of ergosterol in LSC from different origins were determined by HPLC. The conditions were as follows: BDS Hypersil C₁₈ column (250 mm × 4.6 mm, 5 μm); flow rate: 1.0 mL/min; detection wavelength: 282 nm; column temperature: 30 ℃; injection volume: 20 μL; mobile phase: acetonitrile (A): 0.1% formic acid (B) as the mobile phase gradient elution: 0—15 min, 95% A, 15—50 min, 100% A. Results The HPLC method to determine the ergosterol content of puffball was established.The ranges of ergosterol contents among different samples was 0.009 9%—0.150 3%. Conclusion The contents of ergosterol in different LSC are obviously different, and the quality of commercial LSC is not coincident. So the quality control of LSC a needs further normalization and standardization. The data in this paper would provide a reasonable reference for the quality control of LSC.

河北省科技计划项目(12277794)