目的 比较过路黄Lysimachia christinae和广金钱草Desmodium styracifolium的化学成分异同,为阐明二者药效异同提供参考。方法 采用RP-HPLC建立过路黄和广金钱草的HPLC-UV指纹图谱和HPLC-ELSD指纹图谱,比较二者2种指纹图谱的差异。结果 过路黄的HPLC-UV指纹图谱和其HPLC-ELSD指纹图谱的差异很大,UV检测到的强峰并非ELSD检测到的强峰,广金钱草亦然;过路黄的HPLC-UV指纹图谱有14个共有峰,HPLC-ELSD指纹图谱有5个共有峰;而广金钱草的HPLC-UV指纹图谱有28个共有峰,HPLC-ELSD指纹图谱有18个共有峰;13批过路黄的指纹图谱相似度较13批广金钱草的相似度差;过路黄和广金钱草的HPLC-UV指纹图谱有4个共有峰,二者的HPLC-ELSD指纹图谱有2个共有峰。结论 HPLC-ELSD指纹图谱能更好地反映过路黄和广金钱草中量较高的非挥发性成分组成的全貌;市售过路黄的质量差异大,而市售广金钱草的质量稳定;过路黄和广金钱草可能含有2种量较高的相同成分,作为二者治疗相同病症的物质基础,但该2种药材应该各具偏性,各有独特的治疗功用,应明确区分。

Objective The differences in the compounds between Lysimachia christinae and Desmodium styracifolium were compared in order to provide the references for pharmacodynamic differences of them.Methods The RP-HPLC-UV and RP-HPLC-ELSD fingerprints of L.christinae and D.styracifolium were established, and the common peaks of two kinds of fingerprints were compared.Results The HPLC-UV fingerprints of L.christinae were obviously different from the HPLC-ELSD fingerprints, and the strong peaks detected in UV were not the same as what detected in ELSD, so were those of D.styracifolium.There were 14 and 5 common peaks, respectively in the HPLC-UV and HPLC-ELSD fingerprints of L.christinae, while 28 and 18 common peaks, respectively in the HPLC-UV and HPLC-ELSD fingerprints of D.styracifolium.The similarities of chromatograms of D.styracifolium were better than those of L.christinae.Four common peaks were identified in the HPLC-UV fingerprints of L.christinae and D.styracifolium, and two common peaks in their HPLC-ELSD fingerprints.Conclusion It is better using HPLC-ESLD to establish the fingerprints of L.christinae and D.styracifolium.The chemical constituents of L.christinae were significantly different between the different batches.However, little variation was found in the chromatograms between the different batches of D.styracifolium.The two plants may contain two same compounds with high content, which may have the function as the substantial bases of their treatments for the same indication.However, they may have their special efficacies.

国家级大学生创新创业训练计划项目(201410635062)