目的 以不同产地的14批连翘Forsythia suspensa为材料,运用RAPD技术对其进行遗传多样性研究并构建DNA指纹图谱。方法 采用RAPD技术,从45条RAPD随机引物中进行筛选,以14批连翘进行DNA指纹图谱的分析研究。结果 从45条RAPD随机引物中筛选出11条多态性好的引物,利用这11条RAPD引物进行PCR扩增,共获得80条谱带,平均每个引物扩增出7.27条谱带,其中多态性谱带67条,多态性条带比率为83.8%,是连翘药材资源研究的一种有效分子标记。14批连翘药材间的遗传相似系数(genetic similarity,GS)在0.4875~0.9625,表明遗传多样性比较丰富。聚类结果显示遗传多样性与连翘药材来源地有明显的相关性,而且与其亲缘关系较近有关,符合植物种群分布的一般规律。结论 以14批不同产地连翘药材资源的RAPD扩增谱带为基础,构建连翘药材资源的DNA指纹图谱并进行聚类分析。

Objective RAPD technique has been used to construct DNA fingerprints and analyze the genetic diversity in 14 batches of Forsythia suspensa from different origins.Methods Primers with good polymorphism were screened from 45 RAPD primers combinations by PCR, and the DNA fingerprints for 14 batches of F.suspensa were constructed by RAPD technique.Results Eleven RAPD primers with good polymorphism were screened from 45 RAPD primers by PCR amplification, and 80 bands were amplified by the 11 RAPD primers with average 7.27 bands for each primer, of which 67 were polymorphic and PPB was 83.8%.The genetic similarity(GS) ranged from 0.4875 to 0.9625 in 14 batches of F.suspensa from different origins, which showed they had rich genetic diversity.The result of cluster analysis showed that F.suspensa was correlated with sample origin and was consistent with species distribution.Conclusion The DNA fingerprints for 14 batches of F.suspensa are established based on the RAPD amplified bands.

山西省科技攻关项目(20120313015-4)