目的 探究肾脑复元汤(SFD)联合人脐带间充质干细胞(hUC-MSCs)移植对缺血性脑卒中的神经保护作用及机制。方法 采用大脑中动脉栓塞(MCAO)法制备脑缺血再灌注大鼠模型,大鼠随机分为假手术组、模型组、SFD组(11.6 g/kg)、hUC-MSCs组、联合组(SFD+hUC-MSCs),各组根据处死时间随机分为造模4、8、15 d 3个时相组;采用神经功能缺损评分量表评估各组大鼠神经功能改变,HE染色观察海马区脑组织病理改变,免疫组化和Werstern blotting分别检测脑源性神经营养因子(BDNF)和碱性成纤维细胞生长因子(bFGF)的表达。结果 与模型组比较,SFD组、hUC-MSCs组和联合组海马区神经细胞坏死均明显减轻,SFD组(第8、15天)、hUC-MSCs组及联合组(第4、8、15天)神经功能缺损评分显著降低、BDNF和bFGF表达显著升高(P<0.01、0.05)。与SFD组和hUC-MSCs组比较,联合组第8、15天时神经功能缺损评分显著降低、BDNF和bFGF表达显著升高(P<0.05)。结论 SFD联合hUC-MSCs移植能显著改善脑缺血后大鼠神经功能恢复,其作用机制可能与促进脑缺血后神经营养因子BDNF和bFGF表达有关。

Objective To investigate the neuroprotective effects and mechanisms of Shennao Fuyuan Decoction(SFD) combined with implantation of human umbilical cord mesenchymal stem cells(hUC-MSCs).Methods Rats were randomly divided into Sham operation group, model group [cerebral ischemia-reperfusion(I/R) model of rats prepared by middle cerebral artery occlusion, MCAO], SFD group(11.6 g/kg), hUC-MSCs group, combination group(SFD+hUC-MSCs), and these groups were separately divided into other three groups by execution time(days 4, 8, and 15).The neurological function of rats was evaluated by neurological severity scores(NSS), the pathological changes of hippocampus were observed by HE staining, and the expression of BDNF and bFGF was detected by immunohistochemical and Werstern blotting method.Results After cerebral ischemia, the neural necrocytosis of hippocampus in SFD group, hUC-MSCs group, and combination group was alleviated compared with model group;Compared with model group, the NSS was much lower and the expression of BDNF and bFGF was higher in SFD group(days 8 and 15), hUC-MSCs group, and combination group(days 4, 8, and 15)(P<0.01 and 0.05);Compared with SFD group and hUC-MSCs group, the NSS was lower and the expression of BDNF and bFGF was higher in combination group(days 8 and 15)(P<0.05).Conclusion The combination of SFD and hUC-MSCs implantation could significantly improve the restoration of nuerological defects after cerebral ischemia of rats, and this neuroprotective effect is probably due to the promotion of BDNF and bFGF expression.

国家自然科学基金资助项目(81273751,81573941);中西医结合心脑疾病防治湖南省重点实验室项目(2014XNFZ01);湖南省研究生科研创新项目(CX2014B348);湖南省中医药科研计划项目基金(201235)