目的 建立女贞子生品及其炮制品的HPLC指纹图谱。方法 采用Purospher^® STAR RP₁₈ Endcapped(250 mm×4.6 mm,5μm)色谱柱,以水-乙腈为流动相,梯度洗脱,体积流量1.0 mL/min,检测波长282 nm,柱温25℃;采用《中药色谱指纹图谱相似度评价系统》(2012.130723版)软件进行分析。结果 优化了HPLC色谱条件,在女贞子生品及其炮制品的谱图中分别标定了19个共有指纹特征峰,确定了红景天苷、女贞苷、特女贞苷、橄榄苦苷、oleonuezhenide 5个化合物,建立了10个不同批次的女贞子生品及炮制品HPLC指纹图谱,相似度高。结论 该方法快速、简便、准确,符合指纹图谱技术要求,可作为控制女贞子质量的分析方法。

Objective To set up an analysis method of chromatographic fingerprint for crude and processed Ligustri Lucidi Fructus by HPLC.Methods Purospher^® STAR RP₁₈ Endcapped column(250 mm×4.6 mm, 5μm) was used for separation.The separation process was carried out using a gradient elution, which was based on a mobile phase of the mixture aqueous solution with wateracetonitrile at the flow rate of 1.0 mL/min.The detection wavelength was 282 nm and the column temperature was 25℃.The chromatographic fingerprints were analyzed with the Similarity Evaluation System for Chromatographic Fingerprint of TCM(2012.130723 Edition).Results Totally 19 common fingerprint peaks and five constituents including salidroside, privet glycosides, especially privet glycoside, oleuropein, and oleonuezhenide were identified after optimization of the detection wavelength, mobile phase, and gradient elution conditions.Furthermore, the similarity of HPLC fingerprints of 10 batches of Ligustri Lucidi Fructus was high, being over 0.9.Conclusion The present methodology meets the technical requirements of fingerprint with the rapid, simple, and accuracy properties and could be a basis for the intrinsic quality control standard for Ligustri Lucidi Fructus.

科技部"国家科技重大专项"资助项目(2012ZX09304005);江苏高校优势学科建设工程资助项目(PAPD);江苏高校品牌专业建设工程一期项目(PPZY2015A070)