目的 对革叶山姜Alpinia coriacea全草的化学成分进行研究。方法 采用硅胶、ODS、Sephadex LH-20柱色谱及制备型HPLC等色谱方法进行分离纯化,根据波谱数据和理化性质鉴定化合物的结构。结果 从革叶山姜90%乙醇提取物中分离得到15个化合物,分别鉴定为对羟基苯甲醛(1)、4-甲氧基苯甲酸(2)、乔松素(3)、芹菜素(4)、良姜素(5)、山柰酚(6)、5,7,3',4'-四羟基二氢黄酮(7)、3,5-二羟基-7,4'-二甲氧基黄酮(8)、α-tocospiro A(9)、6α-羟基豆甾-4-烯-3-酮(10)、豆甾-4-烯-3-酮(11)、7-酮基-β-谷甾醇(12)、5α,8α-表二氧-(22E,24R)-麦角甾-6,22-二烯-3β-醇(13)、豆甾醇(14)和β-谷甾醇(15)。结论 所有化合物均为首次从革叶山姜中分离得到,其中化合物2、7、9~13为首次从山姜属植物中分离得到。

Objective To study the chemical constituents of Alpinia coriacea.Methods The chemical constituents were separated and purified by silica gel, ODS, Sephadex LH-20 column chromatographies, and preparative HPLC.Their structures were determined by physicochemical properties and spectral data analyses.Results Fifteen compounds were isolated from A.coriacea, and identified as 4-hydroxybenzaldehyde(1), anisic acid(2), pinocembrin(3), apigenin(4), izalpinin(5), kaempferol(6), 5, 7, 3', 4'-tetrahydroxyflavanone(7), 3, 5-dihydroxy-7, 4'-dimethoxyflavone(8), α-tocospiro A(9), stigmast-4-ene-6α-ol-3-one(10), stigmast-4-en-3-one(11), 7-keto-β-sitosterol(12),(22E, 24R)-5α, 8α-epidioxyergosta-6, 22-dien-3β-ol(13), stigmasterol(14), and β-sitosterol(15).Conclusion All compounds are isolated from A.coriacea for the first time, among which compounds 2, 7, and 9-13 are isolated from the plants of Alpinia L.for the first time.

国家国际科技合作专项(ISTCP)(2014DFA40850);国家自然科学基金资助项目(81360478,21362009,81160391)