目的 对皂角刺Gleditsiae Spina中的黄酮类成分进行分离纯化和肿瘤细胞毒活性研究。方法 采用硅胶、Sephadex LH-20、ODS柱色谱法和HPLC制备色谱等多种技术对皂角刺黄酮类化学成分进行分离纯化,并通过理化性质和MS、NMR、圆二色谱等谱学数据鉴定化合物的结构。结果 从皂角刺中分离得到12个二氢黄酮醇类及黄酮类化合物,分别鉴定为(2R,3R)-5,3',4'-三甲氧基-7-羟基二氢黄酮醇(1)、5,7,3',4'-四羟基二氢黄酮醇(2)、5-甲氧基-3',4',7-三羟基二氢黄酮醇(3)、二氢山柰酚(4)、表儿茶素(5)、5,7,3',5'-四羟基二氢黄酮醇(6)、黄颜木素(7)、(2R,3R)-7,3',5'-三羟基-二氢黄酮醇(8)、(2R,3R)-5,7,3'-三羟基-4'-甲氧基-二氢黄酮醇(9)、槲皮素(10)、5,7,4'-三羟基黄酮-8-C-葡萄糖苷(11)、2,7-二甲基-氧杂蒽酮(12)。结论 化合物1为新化合物,命名为皂角刺二氢黄酮醇A;化合物3、8、9和12均为首次从皂角刺中分离得到。对肿瘤细胞毒活性研究结果显示,二氢黄酮醇类化合物1、2、3、7及黄酮类化合物10均表现出较强的细胞毒活性。其中化合物1和3主要作用于肝癌HepG2和食管癌EC109细胞株,化合物2主要作用于食管癌EC109细胞株,而化合物7则对肝癌、肺癌和胃癌肿瘤细胞均有较强的增殖抑制活性。

Objective To study the flavonoids constituents from the thorns of Gleditsia sinensis and their cytotoxicity against tumor cells. Methods The compounds were isolated by silica gel, Sephadex LH-20 column chromatography, and HPLC techniques. Their structures were elucidated on the basis of spectroscopic analyses. Results Twelve flavonoids were obtained and identified as(2R, 3R)-5, 3', 4'-trimethoxyl-7-hydroxyl-flavanonol(1), 5, 7, 3', 4'-tetrahydroxyl-flavanonol(2), 5-methoxyl-3', 4', 7-trihydroxyl-flavanonol(3), dihydrokaempferol(4), epicatechin(5), 5, 7, 3', 5'-tetrahydroxyl-flavanonol(6), fustin(7),(2R, 3R)-7, 3', 5'-trihydroxyl-flavanonol(8),(2R, 3R)-5, 7, 3'-trihydroxyl-4'-methoxyl-flavanonol(9), quercetin(10), 5, 7, 4'-trihydroxylflavone-8-C-glucopyranose(11), and 2, 7-dimethyl-xanthone(12), respectively. Conclusion Compound 1 is a new component named G. spina flavanonol A, and compounds 3, 8, 9, and 12 are isolated from the thorns of G. sinensis for the first time. The results of cytotoxicity test show that the dihydroflavonol compound 7 displays the stronger cytotoxicity against HepG2, A549, and EC109 cell strains, while compounds 1 and 3 have the effects on HepG2 and EC109, and compound 2 has the effect on EC109 cancer cells, respectively.

国家自然科学基金青年基金(U1504830);教育部留学回国人员科研基金资助项目(13530001);洛阳市科技攻关项目(2014010161)