目的 采用D-最优设计响应面法结合UHPLC技术优选补骨脂药材炮制工艺。方法 以补骨脂药材炮制工艺的关键参数闷润时间、炒制温度及加盐量为自变量,进行D-最优设计;以UHPLC检测获得的补骨脂药材中9种主要指标成分补骨脂素、异补骨脂素、新补骨脂异黄酮、补骨脂甲素、补骨脂定、补骨脂乙素、补骨脂宁、补骨脂查耳酮和补骨脂酚炮制前后的质量分数变化为因变量,进行响应面分析;并结合单因素试验优化炒制时间,优选出补骨脂药材炮制工艺。结果 补骨脂药材的最佳炮制工艺为在100 g补骨脂药材中加入2.10 g盐,闷润12 h,在80 ℃炒制30 min。结论 利用D-最优设计响应面法结合UHPLC能够优选补骨脂药材炮制工艺参数,提高炮制工艺的稳定性和重复性,为提高补骨脂盐炙品的质量均一性和临床用药安全性提供技术支持。

Objective To optimize the processing technology for Psoraleae Fructus by D-optimal response surface methodology with UHPLC. Methods Based on the content variations of nine main constituents (psoralen, isopsoralen, neobavaisoflavone, bavachin, psoralidin, corylifolinin, corylin, bavachalcone, and bakuchiol) determined by UHPLC, the D-optimal response surface methodology combined with single factor experiment was used to optimize the moistened time, stir-fried temperature, salted dosage, and stir-fried time in the processing technology for Psoraleae Fructus. Results The optimal processing technology for Psoraleae Fructus was as follows: 100 g Psoraleae Fructus was mixed with 2.10 g salt, moistened for 12 h, and stir-fried for 30 min at 80 ℃. Conclusion The processing technology for Psoraleae Fructus optimized by D-optimal response surface methodology with UHPLC could be used to optimize the process parameters, to promote the stability and repeatability of the processing technology, which provides the technique support for improving the quality uniformity of Psoraleae Fructus and safety in clinic application.

重庆市“121”科技支撑示范工程项目(cstc2012jcsf-jfzhX0029);重庆市基本科研业务费计划项目(cstc2015ja15-24);重庆市卫生局中医药科技项目(ZY20132068)