目的 建立白芍UPLC指纹图谱测定方法,对不同产地白芍的质量进行较全面的评价。方法 色谱柱ACQUITY UPLC^® HSS T3(100 mm×2.1 mm,1.8 μm),以乙腈-0.05%磷酸水溶液为流动相进行梯度洗脱,检测波长230 nm,柱温30 ℃,体积流量0.4 mL/min。采用相似度评价、聚类分析、主成分分析3种方法对23批白芍饮片指纹图谱进行研究。结果 建立了白芍饮片的专属性UPLC指纹图谱,确定了8个共有峰;23批白芍样品质量差异较大,不同产地间和同一产地内样品均具有一定差异。结论 该方法简便、易行,为白芍饮片的质量控制提供了较为有效的快速评价方法。

Objective To establish a UPLC fingerprint method of Paeoniae Alba Radix, and provide comprehensive evaluation of their quality in different regions. Methods The UPLC chromatographic column was Acquity UPLC^® HSS T3 (100 mm ×2.1 mm, 1.8 μm). The mobile phase was acetonitrile-0.05% phosphoric acid water with gradient elution. The detection wavelength was 230 nm and column temperature was 30 ℃ with the flow rate of 0.4 mL/min. Similarity analysis, hierarchical clustering analysis, and principal component analysis were undertaken to study 23 sets of UPLC fingerprints of Paeoniae Alba Radix. Results A specific UPLC fingerprint of Paeoniae Alba Radix was established and eight common peaks were designated. The results showed that the qualities of the 23 sets of Paeoniae Alba Radix were not stable and the samples collected from same region and different regions both had certain differences. Conclusion UPLC fingerprint is an available and convenient method which can be used to access the quality of Paeoniae Alba Radix rapidly.