目的 研究白头翁水提物(AEPC)对链脲佐菌素(STZ)诱导大鼠2型糖尿病模型的降血糖作用。方法 传统水提法获得AEPC;口服葡萄糖耐量试验(OGTT)初步检测AEPC的降血糖效果;STZ诱导新生大鼠2型糖尿病模型,观察AEPC的降血糖效果,并检测血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)、尿素、三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、总蛋白及胰岛素水平。RIN 5F细胞胰岛素释放实验探究AEPC抗糖尿病的作用机制。结果 OGTT结果显示AEPC具有很好的降血糖效果,并且最优剂量为20 mg/kg;对新生大鼠2型糖尿病模型,20 mg/kg的AEPC处理20 d后表现出明显的降血糖效果,肝糖原水平及其他血清生化指标均恢复正常,与正常大鼠无差异;RIN 5F细胞胰岛素释放实验结果显示AEPC明显促进RIN 5F细胞胰岛素释放。结论 AEPC具有降血糖的效果,其作用机制与促进胰岛β细胞释放胰岛素有关。

Objective To study the blood glucose lowering activity of aqueous extract from Pulsatilla chinensis (AEPC) on Streptozocin (STZ)-induced type-2 diabetic rats. Methods AEPC was obtained through traditional aqueous extraction procedure;Oral glucose tolerance test was employed for the preliminary study of AEPC's blood glucose lowering activity;For further study, STZ-induced neonatal diabetic rat model was used, and the serum SGPT, SGOT, ALP, serum urea, triglycerides, total cholesterol, HDLC, total protein, and serum insulin were tested;The Kunming rats were used for acute toxicity experiments, with the highest dose of 1 000 mg/kg body weight;Insulin release from RIN 5F cells was performed to study the mechanism of AEPC on antidiabetes effect. Results Oral glucose tolerance test showed AEPC could lower the blood sugar, and the optimal activity was observed at a dose of 20 mg/kg body weight;In neonatal STZ-induced type-2 diabetic rats, treatement with 20 mg/kg AEPC for 20 d resulted in significant blood glucose lowering activity, liver glycogen and serum biological parameters back to normal;The acute toxicity results showed that no obvious toxicity was observed even at 1 000 mg/kg dose;At last, AEPC could promote the secretion of insulin from RIN 5F cells. Conclusion AEPC shows the anti-diabetes activity on STZ-induced type-2 diabetic rats, which may be the result of its effect on promoting insulin release from pancreatic α-cells.