目的 探讨白花蛇舌草乙醇提取物(EEHDH)治疗慢性溃疡性结肠炎(UC)及抑制肠炎组织异常增生的作用。方法 小鼠随机分为对照组,模型组,EEHDH低、中、高剂量(2.5、10.0、20.0 g/kg)组,葡聚糖硫酸钠(DSS)诱导UC模型,每天记录小鼠体质量、便血、便形等症状改变,HE染色评估小鼠结肠黏膜组织病理改变,ELISA和实时荧光定量PCR(RT-PCR)检测血清及肠组织中炎症因子白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的表达变化,免疫组化分析肠上皮细胞中核抗原Ki67阳性率。结果 与模型组比较,EEHDH中、高剂量组小鼠体质量下降相对缓慢,停止DSS刺激后恢复也较快;同时小鼠腹泻、便血症状改善明显,病理损伤和肠组织异常增生显著降低(P<0.05、0.01);小鼠血清及肠组织中IL-6、TNF-α的表达及Ki67阳性率显著降低(P<0.05、0.01)。结论 EEHDH能够有效改善DSS诱导的UC症状,并通过抑制IL-6等炎症因子表达降低肠组织的病理损伤、炎症浸润及异常增生,EEHDH对UC的治疗及肠炎相关肿瘤的预防具有重要的价值。

Objective To evaluate the effects of ethanolextract of Hedyotis Diffusae Herba (EEHDH) on chronic ulcerative colitis (UC) and the inhibition on intestinal tissue abnormalities hyperplasia. Methods The C57 mice were randomly divided into five groups: normal, DSS, low-, mid-, and high-dose (2.5, 10.0, and 20.0 g/kg) EEHDH groups. The DSS-induced UC model was employed. The body weight, hematochezia, and stool property were recorded to evaluate the change of physiology daily. The pathology changes of colonic mucosa tissue of mice were evaluated by HE staining, and the expression changes of inflammatory factors IL-6 and TNF-α in serum and tissues were detected by ELISA and real-time PCR, as well as immunohistochemistry was used to analyze the positive rate of Ki67 of the nuclear antigen in epithelial cells of colonic tissues. Results Compared to the model group, the treatment with mid- and high-dose EEHDH has a relatively slower weight loss and a faster recovery when the stimulation is gone;Meanwhile the symptoms of diarrhea and hematochezia are significantly improved, the pathological damage and intestinal tissue dysplasia are alleviated;The inflammatory factors IL-6, TNF-α, and the positive rate of Ki67 are significantly decreased. Conclusion EEHDH could attenuate DSS-induced UC and decrease pathological injury, inflammation invasion, and abnormalities hyperplasia, by inhibiting the expression of inflammatory cytokines, such as IL-6 and TNF-α, which indicates that EEHDHis of great value in the treatment of UC and colitis-associated cancer.

国家自然科学基金资助项目(81274026)