目的 采用絮凝技术优化消炎退热颗粒原药水提液除杂净化工艺,保留有效成分的同时去除更多杂质,提高原药水提液的澄清度。方法 明确消炎退热颗粒原药水提液中杂质的成分后,通过筛选絮凝剂的实验,确定了采用壳聚糖絮凝剂对原药水提液进行絮凝除杂。针对以消炎退热颗粒原药水提液中有效成分秦皮乙素的保留率,杂质成分蛋白质和鞣质的去除率以及絮凝处理后药液上清液的浊度作为研究指标,分别研究考察了壳聚糖絮凝剂的配比及用量、絮凝温度、絮凝快搅速度及快搅时间对絮凝整体效果的影响。结果 通过实验得出壳聚糖作为絮凝剂用于消炎退热颗粒原药水提液除杂净化的最佳絮凝工艺条件为壳聚糖加入量1.25 g/L,絮凝温度为40 ℃,快搅速度为350 r/min,快搅时间为3 min。该条件下有效成分秦皮乙素的保留率为88.92%,蛋白质和鞣质的去除率分别为62.96%和67.19%,絮凝后药液静置24 h后浊度为5.0 NTU。结论 壳聚糖作为絮凝剂用于消炎退热颗粒原药水提液的除杂净化,能有效提高药液中杂质的去除率,同时能提高药液的澄清度。
Objective To optimize the purifying process of water-extraction solution of Xiaoyan Tuire Granules Oral Liquid by flocculation clarifying method, reserve more active components while less impurities, and improve the clarity of oral liquid. Methods After clearing out the impurities composition of water-extraction solution of Xiaoyan Tuire Granules Oral Liquid, the flocculant of chitosan chosen by screening test was used to analyze the flocculation effect. The retention rate of aesculetin, the removal rate of impurity, and supernatant turbidity were set as mainly indexes to estimate the influence of the dosage of flocculant, temperature, fast mixing speed, and fast mixing time. Results The optimal flocculating process conditions which were analyzed by experiments were as follows: the dosage of chitosan was 1.25 g/L, the flocculation temperature was 40 ℃, the fast mixing speed was 350 r/min, and the fast mixing time was 3 min. Under these conditions, the retention rate of aesculetin was 88.92%, the removal rates of protein and tanning were 62.96% and 67.19%, the supernatant turbidity was 5.0 NTU after the oral liquid was flocculated for 24 h. Conclusion Using chitosan as flocculant in the water-extraction solution of Xiaoyan Tuire Granules Oral Liquid, it could improve the removal rate of impurities effeitively as well as keep higher active components and enhance the clarity of oral liquid.