目的 研究菊苣HPLC指纹图谱与其降尿酸药效的相关性,初步揭示菊苣降尿酸药效物质基础。方法 高嘌呤饮食诱导鹌鹑高尿酸血症,并给予菊苣水提物。采用偏最小二乘(PLS)法将菊苣HPLC指纹图谱中各共有峰的相对峰面积和降尿酸药效进行数据处理,研究谱效相关性,确定菊苣降尿酸作用的药效物质基础。结果 菊苣地上部分具有较好的降尿酸药效,根据变量投影重要性指标确定了菊苣HPLC指纹图谱中各个共有峰对降尿酸作用的贡献度。其中,绿原酸、菊苣酸及峰3、6、7、8所代表的化合物对降尿酸药效的贡献度较大。结论 通过菊苣HPLC指纹图谱与其降尿酸药效PLS法分析可知,菊苣降尿酸作用与其所含的多种成分有关。

Objective To study the relationships between HPLC fingerprint of Cichorium intybus extract and the uric acid lowering efficacy and to reveal the material basis of C. intybus. Methods Quail were used in the experiment and high purine diet was used to induce hyperuricemia;In addition, the quail were administered with C. intybus water extract. The partial least squares (PLS) method was used to study the spectrum-effect relationships and to find out the material basis of uric acid lowering efficacy. Results The aerial part of C. intybus has good uric acid lowering effect, and the contribution of various components in C. intybus with uric acid lowering effect was determined according to the variable importance in projection value. Among them, chlorogenic acid, chicory acid, and peaks of 3, 6, 7, and 8 have lager contribution degree than others. Conclusion The PLS analysis on the spectrum-effect relationships indicates that uric acid lowering effect of C. intybus is related to the various components in C. intybus.

国家自然科学基金资助项目(81403152);教育部高等学校博士学科点专项科研基金(20130013120001,20120013130002);北京中医药大学青年教师专项自主课题(2013-QNJSZX008);北京中医药大学科研创新团队(2011-CXTD-014)