目的 研究木豆叶提取物对大鼠心肌缺血再灌注损伤的保护作用。方法 采用结扎冠状动脉前降支方法制备大鼠心肌缺血再灌注损伤模型,缺血前10 min大鼠ig给予不同剂量木豆叶提取物(125、200、500 mg/kg),复方丹参滴丸为阳性对照,再灌注30 min后以II导联心电图监测各组大鼠心律失常出现和持续时间,进行心律失常评分;再灌注90 min 后测定心肌梗死范围,HE染色观察心肌形态学变化。结果 模型组大鼠心律失常出现较早,持续时间较长,室性早搏、室速、室颤等发生率较高,且心律失常在缺血再灌注损伤末期仍非常明显。与模型组相比,木豆叶提取物能够降低大鼠缺血性心律失常的严重程度和发生率,明显缩小心肌梗死范围,减轻心肌细胞肿胀和炎性细胞浸润。结论 木豆叶提取物对心肌缺血再灌注损伤大鼠有一定的保护作用。

Objective To investigate the protective effects of pigeonpea leaves on myocardial ischemia reperfusion injury in rats. Methods A model of myocardial ischemia reperfusion was established by ligating the anterior descending coronary arteries. Rats were administrated with extract from pigeonpea leaves (125, 200, and 500 mg/kg) before ischemia, and the size of myocardial infarction, arrhythmia score, and morphological changes of cardiomyocytes were determined. Results Compared with the model group, pigeonpea leaves could reduce arrhythmia severity and incidence (P <0.05 or 0.01) and the size of myocardial infarction (P <0.05). Pigeonpea leaves could ameliorate the pathological lesion. Conclusion Pigeonpea leaves have the protective effects on myocardial ischemia reperfusion injury in rats.

国家自然科学基金青年科学基金资助项目(81303262);山西中医学院博士科研启动基金;山西中医学院方药药效及其作用机制研究重点科技创新团队(20150401)