目的 采用网络药理学方法对三拗汤治疗哮喘的功效成分及作用机制进行分析,探讨多成分-多靶点-多通路的网络关系。方法 检索近20年文献中治疗哮喘疗效确切的方剂,分析具有代表性配伍关系组方的功效成分,依据PharmMapper数据库构建多成分-蛋白网络,获取的靶点信息利用博奥数据库MAS3.0系统得到相应通路,通过Cytoscape软件建立成分-靶点-通路网络模型。结果 文献检索发现三拗汤组成麻黄、杏仁、甘草为现代哮喘方剂中主要的配伍。通过成分-通路-靶点网络分析表明,三拗汤11个主要功效成分作用于76个靶点,涉及免疫-内分泌-信号传导-细胞过程中如T细胞受体、PPAR、ErbB等46条通路。结论 三拗汤组成药物的功效成分既有共同的作用靶点及通路,又各有侧重,为明确药物之间的配伍关系和探讨哮喘方剂的作用机制提供了参考。

Objective To explore the effective components and mechanism of San-ao Decoction for treating asthma by network pharmacology study, and to investigate the correlations among multi-compounds, multi-targets and multi-pathways. Methods Effective formulaes for asthma in which effect index had been tested were searched in the past 20 years' literature. Major active compounds from the formulae consisted of Chinese medicines with representative compatibility relation were analyzed. The components-proteins networks based on PharmMappear database were built. The targets information access was used to construct and visualize components-targets-pathways network model using Boao pathway database and Cytoscape software. Results and Conclusion The results showed that Ephedra sinica, Armeniacae Amarum Semen, and Glycyrrhiza uralensis which consisted San-ao Decoction were the major compatibility in formulaes for asthma. The 11 active components in San-ao Decoction involved 76 targets and 46 pathways. San-ao Decoction not only had common targets and pathways, but also had their own emphasis. This reflected the formulae effect mode of multi-components, multi-targets, and multi-pathways. It may provide references to deeper study of compatibility relation and mechanism of formulaes for asthma.

国家自然科学基金资助项目(81473580,81072748)