目的 基于超分子印迹模板自主识别理论,以茜草素为印迹模板功能单体进行猪不同组织的体外亲和色谱实验,验证茜草素归肝经机制,为创立中药归经体外研究方法奠定基础。方法 将猪的肝、肺、舌、腹部肌肉、腿部肌肉等组织作固定相制成色谱柱,以PBS为流动相,进行归肝经的茜草素与归脾经的白砂糖(阴性对照)体外亲和色谱实验,采用HPLC测定各洗脱液中茜草素的量,UV法测定阴性对照组各洗脱液中葡萄糖的量,采用总量统计矩分析法获得各亲和色谱参数,获得色谱方程。结果 茜草素在肝、肺、舌、腹部肌肉、腿部肌肉的色谱参数为C₀:0.250 9～4.813 mg/mL;AUC:2.509～48.13 mg;V_R:20.42～30.77 mL;σ²:282.6～532.7 mL²;H:14.13～26.63 mL;n:1.212～1.777;S:0.759 0～1.000。肝脏对茜草素的吸附性最强,腹部肌肉、腿部肌肉、肺、舌对茜草素的吸附作用依次减弱。阴性对照组中,肝脏对葡萄糖的吸附性并非最强。结论 猪的肝器官对茜草素的吸附最大,两者存在超分子印迹模板亲合作用,这与茜草归肝经理论相吻合,由此可建立中药归经的体外初步研究方法。

Objective Based on supramolecular imprinting template self-recognition theory, to lay the foundation for the in vitro channel tropism research methods of Chinese materia medica (CMM). Using alizarin as imprinting template feature monomer, in vitro affinity chromatography of different pig organs was performed to test the mechanism of alizarin belonging to the liver meridian. Methods The liver, lungs, tongue, abdominal muscle, leg muscle groups, and other tissues of pigs, as stationary phases, were made into column chromatography. Using PBS as the mobile phase, in vitro affinity chromatography experiments with alizarin belonging to the liver meridian and white sugar belonging to the spleen meridian were conducted, the content of eluent alizarin was measured by HPLC and the content of sugar was measured by UV. By using the analysis of total statistical moment, the various chromatographic parameters and chromatography equations were obtained. Results The chromatographic parameters for the alizarin in liver, lungs, tongue, abdominal muscles, leg muscles were as following: C₀: 0.250 9—4.813 mg/mL;AUC: 2.509—48.13 mg;V_R: 20.42—30.77 mL;σ²: 282.6—532.7 mL²;H: 14.13—26.63 mL;n: 1.212—1.777;S: 0.759 0—1.000. In the positive control group, the adsorption capacity of liver for alizarin was the strongest, and abdominal, leg, lungs, and tongue were weakened in turn. While in the negative control group, the adsorption capacity of liver for glucose (white sugar) was not the strongest. Conclusion The adsorption capacity of liver for alizarin is the strongest, and liver has an affinity for alizarin by the super molecular imprinting templates, and it matches the theory of meridian belonging to the liver, which would establish the in vitro channel tropism preliminary research methods of CMM.

国家自然科学基金资助项目(81173558,81270055);国家博士点基金项目(20124323110002);教育厅重点项目(14A108);湖南省中医药管理局项目(201494)