目的 制备红景天苷磷脂复合物,并对制备的复合物进行表征。方法 以红景天苷与磷脂的复合率为评价指标,采用单因素试验和正交设计优化复合物制备工艺;采用差示扫描量热分析(DSC)、X射线衍射分析(XRD)和傅里叶变换红外光谱(FT-IR)等方法对红景天苷磷脂复合物进行鉴定。结果 优化的制备工艺为红景天苷与磷脂的投料比1:4,反应物质量浓度为10 mg/mL,反应温度为20 ℃。结论 红景天苷磷脂复合物的形成受药物与磷脂的投料比、药物质量浓度以及反应时间的影响较大,反应温度也有一定的影响,且复合物在形成过程中并未发生结构变化,但与物理混合物有本质的区别。

Objective To prepare salidroside (SAD) phospholipid complex in Rhodiola crenulota and search for its optimum preparation and characterization. Methods Taking the ratio of salidroside and phospholipid as index, the factors that might affect the combination percentage of SAD with PLC (SAD-PLC) were investigated, separately. At the same time, UV, FT-IR, and XRD microscope anaIysis techniques were used to analyze the structure of SAD phospholipid complex. Results In the optimum preparation process of SAD-PLC, tetrahydrofuran was used as reaction solvent, the weight ratio of SAD to phospholipid was 1:4, and the total concentration of SAD and PLC in the reaction solvent was 10 mg/mL, the reaction temperature was 20 ℃. Conclusion The reaction ratio, concentration of SAD, and reaction time have geater impact on the formation of phospholipid complex, and the reaction temperature also has some impact. Chemical changes do not occur in the formation process, but the phospholipid complex is different with the simple physical mixtures.

上海市教委预算内教育教学项目(2013JW24);2014年上海高校教师产学研践习计划(N140239)