目的 建立黄芩Scutellaria baicalensis的UPLC/LTQ-Orbitrap-MS分析测定方法,研究黄芩中黄酮碳苷类成分,并区分其中的同分异构体。方法 采集Orbitrap全扫描的质谱图,并触发动态数据依赖分析模式采集二级、三级和四级的碎片信息,通过图谱解析确定黄酮碳苷的结果,通过比对碎片信息区分同分异构体。结果 在黄芩中共鉴定表征了19个黄酮碳苷类成分,并对其中的同分异构体进行区分。结论 该方法能够准确地筛选黄芩中的黄酮碳苷类成分,并对同分异构体进行区分,为其他中药的成分分析提供参考。

Objective An UPLC-LTQ-Orbitrap-MS spectrometry method has been developed for the efficient separation and detection of C-glycosyl flavonoids in the extract of Scutellaria baicalensis differentiation of isomers. Methods Data were acquired by Orbitrap. MS², MS³, and MS⁴ data were triggered by data-dependent acquisition mode. Isomers of C-glycosyl flavonoids were distinguished by a comparison study of mass distribution. Results A total of 19 compounds were identified as C-glycosyl flavonoids, and several isomers were successfully discriminated. Conclusion This method is proved to be powerful for the identification of C-glycosyl flavonoids and provides the important complementary information for other flavonoid analysis.

国家自然科学基金青年基金(81303295);国家自然科学基金面上项目(81373688);江苏省高校自然科学研究面上项目(13KJB360005)